Patents Assigned to BioFire Defense, LLC
  • Patent number: 10413905
    Abstract: A temperature cycling system (10, 110) is provided for repeatedly heating and cooling a reaction mixture (16). The system (10, 110) includes a first heater (27) and a second heater (28) each movable between a first orientation in which the first or second heater (27, 28) affects the temperature of the reaction mixture (16) and a second orientation in which the first or second heater (27, 28) does not substantially affect the temperature of the reaction mixture (16). During temperature cycling, the second heater (28) is in the second orientation when the first heater (27) is in the first orientation, and the second heater (28) is in the first orientation when the first heater (27) is in the second orientation.
    Type: Grant
    Filed: January 18, 2017
    Date of Patent: September 17, 2019
    Assignee: BioFire Defense, LLC
    Inventor: Kirk M. Ririe
  • Publication number: 20190046989
    Abstract: Instruments, methods, and kits are disclosed for performing fast thermocycling.
    Type: Application
    Filed: February 21, 2017
    Publication date: February 14, 2019
    Applicant: BIOFIRE DEFENSE, LLC
    Inventors: Kirk Max Ririe, David E. Jones, Christopher Paul Pasko, Anson Cole Chamberlain, Derek David, Aaron Wernerehl, Jonathan Allen Bruns
  • Publication number: 20180372639
    Abstract: A solid fluorescence standard that can be used to calibrate and/or normalize a device (e.g., a scientific instrument) that is configured for generating and collecting fluorescence data. A fluorescence standard disclosed herein includes an adhesive (e.g., a low viscosity, substantially optically transparent, solvent-free, radiation curable adhesive, such as, but not limited to, a UV curable adhesive), and a selected quantity of fluorescent particles (e.g., quantum dots) dispersed in the adhesive. The adhesive and the fluorescent particles are mixed together and disposed in a sample well. The adhesive is then cured and solidified, which yields a solid fluorescence standard in the well.
    Type: Application
    Filed: December 15, 2016
    Publication date: December 27, 2018
    Applicant: BioFire Defense, LLC
    Inventors: Patrick L. RILEY, Richard David ABBOTT, Lyle M. NAY, Sarah M. FOWDEN
  • Patent number: 9850381
    Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
    Type: Grant
    Filed: November 17, 2011
    Date of Patent: December 26, 2017
    Assignees: UNIVERSITY OF UTAH RESEARCH FOUNDATION, BIOFIRE DEFENSE, LLC
    Inventors: Carl T. Wittwer, Gudrun Reed, Virginie Dujols, Luming Zhou
  • Patent number: 9802200
    Abstract: Tools and methods are provided for removing a plurality of tubes from a rack of tubes. Tube rack tools include a base and/or top piece for receiving the rack of tubes. The base has a support surface for engaging the closed bottom of the plurality of tubes. The support surface can be curved such that the rack of tubes can be rocked about the curves support surface to dislodge the plurality of tubes from the rack. The top piece can be configured to engage the rack such that pressure on the top piece in the direction of the support surface applies a pressure on the rack without applying the pressure to the tubes. The tubes are dislodged from the rack by application of the pressure. Elements of the tube rack tool are also configured for securing the tubes within the rack or caps to the tubes.
    Type: Grant
    Filed: February 11, 2016
    Date of Patent: October 31, 2017
    Assignee: BioFire Defense, LLC
    Inventors: Logan M. Taylor, Richard David Abbott
  • Patent number: 9771610
    Abstract: Methods and kits are provided for nucleic acid analysis. In an illustrative method a target nucleic acid is amplified using a first primer and a second primer, wherein the first primer comprises a probe element specific for a locus of the target nucleic acid and a template-specific primer region, and the probe element is 5? of the template-specific primer region, subsequently allowing the probe element to hybridize to the locus to form a hairpin, generating a melting curve for the probe element by measuring fluorescence from a dsDNA binding dye as the mixture is heated, wherein the dye is not covalently bound to the first primer, and analyzing the shape of the melting curve. Kits may include one or more of the first and second primers, the dsDNA binding dye, a polymerase, and dNTPs.
    Type: Grant
    Filed: February 19, 2013
    Date of Patent: September 26, 2017
    Assignees: University of Utah Research Foundation, BioFire Defense, LLC
    Inventors: Carl T. Wittwer, Luming Zhou, Mark Aaron Poritz
  • Patent number: 9657347
    Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
    Type: Grant
    Filed: August 19, 2014
    Date of Patent: May 23, 2017
    Assignee: University of Utah Research Foundation and BioFire Defense, LLC
    Inventors: Luming Zhou, Carl T. Wittwer, Philip Seth Bernard, Virginie Dujols
  • Patent number: 9586208
    Abstract: A temperature cycling system (10, 110) is provided for repeatedly heating and cooling a reaction mixture (16). The system (10, 110) includes a first heater (27) and a second heater (28) each movable between a first orientation in which the first or second heater (27, 28) affects the temperature of the reaction mixture (16) and a second orientation in which the first or second heater (27, 28) does not substantially affect the temperature of the reaction mixture (16). During temperature cycling, the second heater (28) is in the second orientation when the first heater (27) is in the first orientation, and the second heater (28) is in the first orientation when the first heater (27) is in the second orientation.
    Type: Grant
    Filed: July 5, 2013
    Date of Patent: March 7, 2017
    Assignee: BioFire Defense, LLC
    Inventor: Kirk M. Ririe
  • Patent number: 9446410
    Abstract: An apparatus for thermal cycling can transfer heat uniformly and efficiently. The apparatus can be used in a method that reduces condensation on sample wells. The apparatus can also be manufactured to provide uniform configurations. For example, a sample, illustratively for polymerase chain reaction (PCR), in each sample well and the components of the embodiment of the thermal cycler apparatus shown at including a well block, a base plate, a layer of adhesive, a peltier device, another layer of adhesive and a heat sink.
    Type: Grant
    Filed: December 2, 2011
    Date of Patent: September 20, 2016
    Assignee: BIOFIRE DEFENSE, LLC
    Inventors: Zackery Kent Evans, Thomas Knight Bodily, Richard David Abbott, Patrick L. Riley
  • Patent number: 9290663
    Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
    Type: Grant
    Filed: March 8, 2012
    Date of Patent: March 22, 2016
    Assignees: University of Utah Research Foundation, BioFire Defense, LLC
    Inventors: Carl T. Wittwer, Gudrun Reed, Virginie Dujols, Luming Zhou
  • Patent number: D791337
    Type: Grant
    Filed: February 24, 2016
    Date of Patent: July 4, 2017
    Assignee: BioFire Defense, LLC
    Inventors: Logan M. Taylor, Richard David Abbott