Patents Assigned to BioFire Diagnostics, Inc.
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Patent number: 9422597Abstract: Methods are provided for nucleic acid analysis. In an illustrative method, allele amplification bias is used to amplify preferentially a target nucleic acid that is present in a low allele fraction.Type: GrantFiled: November 6, 2009Date of Patent: August 23, 2016Assignee: BioFire Diagnostics, Inc.Inventors: Jason T. McKinney, Luming Zho, Cameron N. Gundry, Robert Andrew Palais
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Patent number: 9273346Abstract: An experimental melting curve is modeled as a sum of a true melting curve and background fluorescence. A deviation function may be generated based upon the experimental melting curve data and a model of a background signal. The deviation function may be generated by segmenting a range of the experimental curve into a plurality of windows. Within each window, a fit between the model of the background signal and the experimental melting curve data may be calculated. The deviation function may be formed from the resulting fit parameters. The deviation function may include background signal compensation and, as such, may be used in various melting curve analysis operations, such as data visualization, clustering, genotyping, scanning, negative sample removal, and the like. The deviation function may be used to seed an automated background correction process. A background-corrected melting curve may be further processed to remove an aggregation signal.Type: GrantFiled: May 14, 2010Date of Patent: March 1, 2016Assignee: BIOFIRE DIAGNOSTICS, INC.Inventors: Thomas Charles Robbins, Robert Andrew Palais, Carl Thomas Wittwer
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Publication number: 20140273181Abstract: Optical systems and apparatuses configured for enabling substantially simultaneous observation of a plurality of points in an array from a common reference point. Without the optical systems and apparatuses disclosed herein, less than all of the plurality of points can be observed substantially simultaneously from the common reference point.Type: ApplicationFiled: March 15, 2013Publication date: September 18, 2014Applicant: BioFire Diagnostics, Inc.Inventors: Richard David Abbott, Patrick L. Riley, Zackery Kent Evans, Lyle M. Nay
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Publication number: 20140051155Abstract: An apparatus for thermal cycling can transfer heat uniformly and efficiently. The apparatus can be used in a method that reduces condensation on sample wells. The apparatus can also be manufactured to provide uniform configurations.Type: ApplicationFiled: December 2, 2011Publication date: February 20, 2014Applicant: BioFire Diagnostics, Inc.Inventors: Zackery Kent Evans, Thomas Knight Bodily, Richard David Abbott, Patrick L. Riley
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Publication number: 20140038272Abstract: A temperature cycling system (10, 110) is provided for repeatedly heating and cooling a reaction mixture (16). The system (10, 110) includes a first heater (27) and a second heater (28) each movable between a first orientation in which the first or second heater (27, 28) affects the temperature of the reaction mixture (16) and a second orientation in which the first or second heater (27, 28) does not substantially affect the temperature of the reaction mixture (16). During temperature cycling, the second heater (28) is in the second orientation when the first heater (27) is in the first orientation, and the second heater (28) is in the first orientation when the first heater (27) is in the second orientation.Type: ApplicationFiled: July 5, 2013Publication date: February 6, 2014Applicant: BioFire Diagnostics, Inc.Inventor: Kirk Max Ririe
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Publication number: 20130171045Abstract: A device is provided for receiving a fluid sample. The device includes a fitment having a cavity formed therein. The cavity is provided under vacuum. The fitment also includes a port having a seal. The port is configured to provide fluid connection from an exterior surface of the fitment to the cavity upon opening of the seal. The device optionally includes a collapsible compartment coupled to the fitment and in fluid communication with the cavity.Type: ApplicationFiled: February 27, 2013Publication date: July 4, 2013Applicant: BioFire Diagnostics, Inc. f/k/a Idaho Technology, Inc.Inventor: BioFire Diagnostics, Inc. f/k/a Idaho Technology, Inc.
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Publication number: 20130157349Abstract: Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include nucleic acid amplification and detection and immuno-PCR.Type: ApplicationFiled: February 12, 2013Publication date: June 20, 2013Applicant: BioFire Diagnostics, Inc., f/k/a Idaho Technology, Inc.Inventor: BioFire Diagnostics, Inc., f/k/a Idaho Technology, Inc.
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Publication number: 20130137172Abstract: Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include high density nucleic acid amplification and detection and immuno-PCR.Type: ApplicationFiled: January 28, 2013Publication date: May 30, 2013Applicant: BioFire Diagnostics, Inc. f/k/a Idaho Technology, Inc.Inventor: BioFire Diagnostics, Inc. f/k/a Idaho Technology, Inc.
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Patent number: 8409508Abstract: A device is provided for receiving a fluid sample. The device includes a fitment having a cavity formed therein. The cavity is provided under vacuum. The fitment also includes a port having a seal. The port is configured to provide fluid connection from an exterior surface of the fitment to the cavity upon opening of the seal. The device optionally includes a collapsible compartment coupled to the fitment and in fluid communication with the cavity.Type: GrantFiled: April 23, 2003Date of Patent: April 2, 2013Assignee: BioFire Diagnostics, Inc.Inventors: Kirk Max Ririe, Brent Farnes Higginson, Abigail Bird
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Patent number: 8399189Abstract: Methods and kits are provided for nucleic acid analysis. In an illustrative method a target nucleic acid is amplified using a first primer and a second primer, wherein the first primer comprises a probe element specific for a locus of the target nucleic acid and a template-specific primer region, and the probe element is 5? of the template-specific primer region, subsequently allowing the probe element to hybridize to the locus to form a hairpin, generating a melting curve for the probe element by measuring fluorescence from a dsDNA binding dye as the mixture is heated, wherein the dye is not covalently bound to the first primer, and analyzing the shape of the melting curve. Kits may include one or more of the first and second primers, the dsDNA binding dye, a polymerase, and dNTPs.Type: GrantFiled: March 7, 2008Date of Patent: March 19, 2013Assignees: University of Utah Research Foundation, BioFire Diagnostics, Inc.Inventors: Carl T. Wittwer, Luming Zhou, Mark Aaron Poritz
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Patent number: 8394608Abstract: Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analysis include nucleic acid amplification and detection and immuno-PCR.Type: GrantFiled: May 8, 2006Date of Patent: March 12, 2013Assignee: BioFire Diagnostics, Inc.Inventors: Kirk M. Ririe, Michael R. Newswander, Randy P. Rasmussen, Mark Aaron Poritz, Stewart Benjamin Smith, David E. Jones, Gary Clark Kessler
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Patent number: 8343754Abstract: The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.Type: GrantFiled: May 7, 2012Date of Patent: January 1, 2013Assignees: University of Utah Research Foundation, BioFire Diagnostics, Inc.Inventors: Carl T. Wittwer, Kirk M. Ririe, Randy P. Rasmussen