Abstract: A method for obtaining a plant having an enriched content of cysteine and/or glutathione, including steps which consist in: transforming at least a plant cell with a vector containing an expression cassette including a sequence coding for an adenosine 5?-phosphosulphate reductase (APR); culturing the transformed cell so as to generate a plant containing in its genome the expression cassette.

Abstract: The subject of the present invention is a method for obtaining transgenic plants expressing a protein with H2O2 producing activity, which comprises the following steps of: (a) transforming plant cells with Agrobacterium rhizogenes containing a vector carrying a gene encoding a protein producing H2O2 in a context which allows its expression in the plant; (b) selecting the transformants which contain and express this gene, by a peroxidase-based colorimetric test; (c) regenerating the plants from the roots selected and monitoring the expression of the plantlets obtained by a peroxidase-based colorimetric test; (d) sorting according to phenotype and optionally carrying out the molecular analysis of the progeny of the transgenic plants, allowing the selection or the confirmation of transgenic plants obtained containing only the transgene and not the T-DNA specific to A. rhizogenes.

Abstract: The present invention relates to nucleic acid sequences encoding tapetum specific promoters, for use in Artificial Male Sterility systems in plants. In particular, the promoters may be the pMAC2 promoter; the pMAC20 promoter; or promoter sequences which naturally controls the expression of a coding sequence substantially homologous to the MAC2 or MAC20 coding sequences. Also provided are regulatory elements of the promoters; plant cells and plants transformed with the promoter sequences.

Abstract: The invention concerns promoter nucleotide sequences enabling expression of encoding sequences whereto they can be bound, which is specific of the endosperm region enclosing the embryo of seeds of Angiosperms and which intervene in particular in the early stages of endosperm development, and their use for agronomic or nutritional improvement of plants.

Abstract: The present invention provides isolated nucleic acid molecules encoding wheat starch syntheses, and probes and primers derived therefrom, which are useful in the modificaiton of plant starch content and/or composition, and for screening plant lines to determine the presence of natural and/or induced mutations in starch synthase genes which affect starch content and/or composition. More particularly the isolated nucleic acid molecules of the present invention further provide for the screening-assisted breeding of plants having desirable starch content and/or composition, in addition to providing for the direct genetic manipulation of plant starch content and/or composition.

Abstract: The preset invention relates to the transformation of maize with a grain softness-enhancing gene for improving corn starch extractability. The invention more particulary concerns the reduction of wet milling time using genetically transformed corn that expresses puroindoline.

Abstract: The present invention relates to a nucleic acid sequence encoding an enzyme of the starch biosynthetic pathway in a cereal plant, wherein the enzyme is selected from the group consisting of starch branching enzyme I, starch branching enzyme II, starch soluble synthase I, and debranching enzyme, with the provisio that the enzyme is not soluble starch synthase I of rice, or starch branching enzyme I of rice or maize.

Abstract: A method for producing a modified glutenin or seed-storage protein, the method comprising adding to the protein an exogenous amino acid domain which confers to the modified protein the ability to bind a ligand or other macromolecule. Wherein the modified protein has an ability to incorporate into gluten, modified glutenin or seed-storage proteins, and uses thereof.

Abstract: The invention concerns a method for obtaining plants producing modified polysaccharides (such as starch or glycogen), said modified polysaccharides extracted from said plants and the products prepared from said modified polysaccharides. The invention also concerns a method for obtaining modified starch or modified glycogen which consists in contacting a starch or a glycogen with an ?-1,4 glucanotransferase enzyme.

Abstract: The present invention relates to inductible promoters for use in the control of heterologous genes in transformed plants. Suitable inducible promoters art those which are responsive to low levels of an environmentally acceptable and non-phytoxic inducing agent, and which also demonstrates a low level of developmentally or environmentally induced expression. A preferred prompter naturally drives the expression of a 21.3 kDa protein in Asparagus officinalis or an equivalent protein from the Lillaceae or Amaryllidaceae families. Under the control of a promoter of the invention, a gene will be expressed upon induction by SA or BTH, but preferably will not be developmentally expressed, systemically activated upon pathogen infection, or in response to ABA, ethylene, oxidative or osmotic stresses, or wounding.

Abstract: Method of production of transgenic plants wholly transformed into To generation. The method consists of a) genetically transforming a meristem explant; b) selective culturing for the specific development, among all the transformed cells, of those cells giving rise to secondary meristems and/or those cells capable of resulting in neoformed foliar meristems, c) regenerating, from the cellular material obtained during step b), transgenic plants.

Abstract: The use of sulphonamide resistance genes as selectable markers in wheat is provided.

Abstract: Plants, particularly transgenic plants, maybe produced having a 2-acyltransferase enzyme or other insoluble acyltransferase enzyme with an altered substrate specificity compared to the native enzyme. For example, oil seed rape (Brassica napus) may contain a 2-acyltransferase transgene derived from Limnanthes douglassi in order to increase the erucic acid content of the oil. The cDNA sequence of maize (Zea mays) 2-acyltransferase is disclosed and is useful for cloning acyltransferase genes and/or CDNAs from other organisms, including L. douglassi.

Abstract: The invention relates to the novel use of a sequence coding for a protein capable of degrading oxalic acid to select plant cells which have integrated a gene of interest, and a novel process for selecting, on oxalic acid, cells, calluses or plants transformed by this recombinant DNA.

Abstract: The invention relates to the use of nucleic acid sequences coding for polygalacturonase in the control of dehiscence in plants. Plants transformed with such nucleic acid sequences are also disclosed.

Abstract: A tapetum-specific callase (.beta.-1,3-glucanase) gene, designated A6, from Brassica napus and other members of the family Brassicaceae including A. thaliana has been discovered, isolated and cloned. The A6 gene encodes a 53 kDa callase enzyme of Brassica napus and equivalent proteins in other Brassicaceae family members. Coding sequence from the gene can be driven by an appropriate promoter to induce male sterility in plants. Further, the A6 promoter can be used to drive male sterility DNA such as that coding for a nuclease, protease or glucanase. Alternatively or in addition, male sterility can be achieved by disrupting the proper expression of the A6 gene, for example by transcribing RNA which is antisense to the RNA normally transcribed from the A6 gene, or by expressing DNA coding for a ribozyme specific for the A6 gene RNA transcript.

Abstract: Plants, particularly transgenic plants, may be produced having a 2-acyltransferase enzyme or other insoluble acyltransferase enzyme with an altered substrate specificity compared to the native enzyme. For example, oil seed rape (Brassica napus) may contain a 2-acyltransferase transgene derived from Limnanthes douglassi in order to increase the erucic acid content of the oil. The cDNA sequence of maize (Zea mays) 2-acyltransferase is disclosed and is useful for cloning acyltransferase genes and/or cDNAs from other organisms, including L. douglassi.

Abstract: Recombinant or isolated nucleic acid sequences which:(a) encode enzymes or other proteins involved in plant abscission or dehiscence;(b) contain a promoter or other regulatory sequence which naturally controls expression of a gene involved in plant abscission or dehiscence;(c) when introduced into a plant, prevent or otherwise interfere with normal plant abscission or dehiscence; or(d) hybridise under stringent conditions to nucleic acid satisfying criterion (a), (b) or (c) or would do so but for the degeneracy of the genetic codeare useful in regulating abscission and dehiscence, particularly in reducing pod shatter in Brassica napus.