Abstract: In accordance with the invention, isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are described. More particularly, nucleic acids isolated from Pichia pastoris wherein the nucleic acids have promoter activity are described. The invention also relates to expression methods, host cells, expression vectors, and DNA constructs, for using the Pichia pastoris promoters to produce proteins and polypeptides, and to the proteins and polypeptides produced using the expression methods.
Type:
Grant
Filed:
January 4, 2023
Date of Patent:
April 1, 2025
Assignee:
BIOGRAMMATICS, INC.
Inventors:
Ilya I. Tolstorukov, James M. Cregg, Thomas G. Chappell, Knut R. Madden
Abstract: Isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are disclosed. More specifically, nucleic acids isolated from Pichia pastons having promoter activity and expression methods, host cells, expression vectors, and DNA constructs of using the Pichia pastons promoters to produce different proteins and polypeptides are disclosed.
Type:
Grant
Filed:
April 29, 2020
Date of Patent:
January 17, 2023
Assignee:
BIOGRAMMATICS, INC.
Inventors:
Ilya I. Tolstorukov, James M. Cregg, Thomas G. Chappell, Knut R. Madden
Abstract: In accordance with the invention, isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are described. More particularly, nucleic acids isolated from Pichia pastoris wherein the nucleic acids have promoter activity are described. The invention also relates to expression methods, host cells, expression vectors, and DNA constructs, for using the Pichia pastoris promoters to produce proteins and polypeptides, and to the proteins and polypeptides produced using the expression methods.
Type:
Grant
Filed:
March 7, 2014
Date of Patent:
June 9, 2020
Assignee:
Biogrammatics, Inc.
Inventors:
Ilya I. Tolstorukov, James M. Cregg, Thomas G. Chappell, Knut R. Madden