Abstract: An enzymatic electrochemical method for the quantification of analytes in isolated samples of biological fluids based on a dual biosensor strip electrochemical system. The sample is analyzed in parallel using first and second different biosensor test strips (blank and complete) and the results are obtained by subtracting a response obtained in the electrodes of the first biosensor test strip from a response obtained in the electrodes of the second biosensor test strip, thus eliminating the effect of interferences and isolating the signal corresponding only to the analyte determination.