Abstract: Process of purification and stabilization of the enzyme Gluconate Dehydrogenase (GADH, EC 1.1.99.3) either recombinant or not, from several microorganisms such as: Pseudomonas aeruginosa, Pseudomonas fluoresoens, Gluconobacter oxydans, Gluconobacter industrius, Serratia maroescens Kiebsielia pneumoniae and Eschericia coli and its use as an element of biological recognition in biosensors for the determination of gluconic acid in samples of interest.

Abstract: The invention relates to a biosensor support comprising a cylinder (1) consisting of a non-electroconductive material and having: a through-hole (2) coaxial to the axis of the cylinder (1); a metal rod (3) arranged in said opening (2), with its upper end (31) level with the top base (11) of the cylinder (1); a layer/coating of noble metal (4) of between 1 and 1000 nanometers, arranged on the top base (11) of the cylinder, and a protector (5) consisting of a non-electroconductive material arranged on the layer/coating of noble metal (4) in the area where the layer (4) joins the metal rod (3), said protector covering a surface having a diameter (Ø2) larger than the diameter of the rod (3). The invention is applicable to the specific detection of substances in aqueous media.

Abstract: The invention relates to a biosensor support comprising a cylinder (1) consisting of a non-electroconductive material and having: a through-hole (2) coaxial to the axis of the cylinder (1); a metal rod (3) arranged in said opening (2), with its upper end (31) level with the top base (11) of the cylinder (1); a layer/coating of noble metal (4) of between 1 and 1000 nanometres, arranged on the top base (11) of the cylinder, and a protector (5) consisting of a non-electroconductive material arranged on the layer/coating of noble metal (4) in the area where the layer (4) joins the metal rod (3), said protector covering a surface having a diameter (Ø2) larger than the diameter of the rod (3). The invention is applicable to the specific detection of substances in aqueous media.