Abstract: The present invention features a coating consisting of a functionalized polymeric surface coating comprising reactive entities. These entities could consist of amino-, hydroxyl; epoxy- or other covalently linked in a polymer network of polyurea and polyurethane to provide a surface coating. This functionalized coating is applied to surfaces of a non-woven material composed of fibers. One embodiment of present invention is application to fibers at a level of 0.01 to 1 micromole/cm2 amine without poragens to be utilized for peptide and combinational chemistry synthesis. Furthermore, the coating can be applied to fiber at a high level with a crinkly fiber coating abased on the use of a poragen, thus creating a matrix having a higher loading on higher surface area for synthesis and other applications. These embodiments may be applied to various fields and technologies, e.g., proteomics, genomics, combinatorial chemistry, and chromatography.

Abstract: The present invention relates to improved alternatives for hot-start PCR hybridization and amplification methods by using, in an embodiment, a heat-reversible, covalent modification of nucleic acids to disrupt hybridization of primer to template, or to interfere with the ability for the polymerase enzyme to recognize nucleoside triphosphates. In an illustrative embodiment, the amino groups of guanosine have been reversibly modified by reaction with glyoxal under mild conditions.

Abstract: Methods for preparing organic compounds, such as oligonucleotides, on solid supports are described. Libraries of the compounds and methods of using the compounds are also disclosed.