Abstract: Apparatus for fractionating components separated in liquid chromatography is disclosed. The apparatus includes a sample applying section, a column section, a detector section, and a tubing line connecting the sections. The apparatus further includes a fractionation tube for fractionating eluent portions containing prospective components separated from the column, an air injector for injecting air into the fractionation tube, and a valve for a switching operation such that either of the eluent fed through a flow path or air fed through an air flow path from the air injector is led into the fractionation tube while the other is drained to the drain side.

Abstract: A method of fractionating components separated in liquid chromatography. The method comprises the steps of applying a sample to a column filled with a separation media while supplying an eluent to the column, causing eluent portions containing components in the sample to flow into a fractionation tube connected to the end of an eluent flow path, holding the eluent portions in the fractionation tube, and injecting air into a rear portion of the eluent portions in the fractionation tube.