Patents Assigned to bioMeriéux, S.A.
-
Patent number: 7807439Abstract: The invention concerns a medium for specific detection of Staphylococcus aureus and/or discrimination between positive-coagulase Staphylococcus and negative-coagulase enabling Staphylococcus to isolate bacteria of the genus staphylococcus and identify the Staphylococcus aureus species, which use at least an enzymatic substrate, preferably a chromogenic or fluorescent agent, and still more preferably consisting of an indoxyl or naphthol base. The invention also concerns a method for identifying, and optionally counting, Staphylococcus aureus using such a medium. It consists of a Staphylococcus aureus culture medium and at least an enzymatic substrate enabling testing of an ?-glucosidase activity. The invention is particularly applicable in the field of diagnosis.Type: GrantFiled: March 29, 2002Date of Patent: October 5, 2010Assignee: Biomerieux S.A.Inventors: Christine Cotte, Sylvain Orenga, Andréa Re, Denis Robichon
-
Patent number: 7741129Abstract: A method for treating magnetic particles present in a solution in a container by redispersion, rinsing or displacement, the particles having novel characteristics under the effect of a specific magnet and being associated or not with biological entities. The magnetic particles undergo at least one low-intensity magnetization where they are disposed in filaments oriented according to the north-south axis of the magnet; the magnetization source and/or container is/are displaced while the magnetic effect is maintained on the magnetic particles; the magnetization source and/or container is/are displaced or the magnetization is stopped, in order to suppress the magnetic field on the magnetic particles. Preferably, the invention can be used in the field of biology.Type: GrantFiled: April 14, 2008Date of Patent: June 22, 2010Assignee: BioMerieux S.A.Inventor: Bruno Colin
-
Patent number: 7741027Abstract: Sequences of nucleic acid oligonucleotides for amplifying different portions of gag and pol genes of HIV-1 and for detecting such amplified nucleic acid sequences are disclosed. Methods of amplifying and detecting HIV-1 nucleic acid in a biological sample using the amplification oligonucleotides specific for gag and pol target sequences are disclosed.Type: GrantFiled: April 9, 2008Date of Patent: June 22, 2010Assignees: Gen-Probe Incorporated, BioMerieux S.A.Inventors: Yeasing Y. Yang, Steven T. Brentano, Odile Babola, Nathalie Tran, Guy Vernet
-
Patent number: 7723095Abstract: A method for lysing prokaryotic or eukaryotic cells, or for simultaneous lysis of both, which includes at least three of the following: a mass of active, small-diameter beads corresponding to 50% or less than a mass of active, larger-diameter beads, and/or a total mass of lysing beads (consisting of a single type of bead or a mixture of smaller and larger beads) corresponding to between 50 and 100% of the total mass of the processed biological sample, and/or a lysis time of from 10 to 20 minutes, and/or seven or less non-lysing glass beads to drive the movement of the lysing beads, and/or from five to fifteen non-lysing iron beads to drive the movement of the lysing beads, depending on whether sonication, mechanical vortex centrifugation or magnetic vortex centrifugation is used.Type: GrantFiled: July 26, 2001Date of Patent: May 25, 2010Assignee: Biomerieux S.A.Inventors: Philippe Cleuziat, Sandra Incardona, Corinne Jay
-
Publication number: 20100062540Abstract: The present invention concerns a method for detecting aggregate-forming circulating protein forms in a biological sample of human origin that may contain said aggregate-forming circulating protein forms, characterized in that it uses a non-protein agent I producing aggregation of the circulating forms of the noninfectious proteins involved in pathological aggregation processes of the central nervous system and/or a non-protein agent II for capturing the natural aggregrates of aggregate-forming circulating protein forms or the aggregates induced by said agents I.Type: ApplicationFiled: July 7, 2006Publication date: March 11, 2010Applicants: BIOMERIEUX S.A., CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE CLAUDE BERNARD LYON 1Inventors: Sébastien Cecillon, Anthony William Coleman, Anne Eveno-Nobile, Hervé Perron, Marc Rodrigue
-
Publication number: 20100047895Abstract: The present invention relates to a method for the selective purification of bacterial cells and/or cell components, whereby the purification is performed by means of a solid support.Type: ApplicationFiled: July 28, 2009Publication date: February 25, 2010Applicants: Hyglos Invest GmbH, bioMerieux S.A.Inventors: Michael Schütz, Renate Grassl, Roman Meyer, Sibylle Frick, Ingrid Robl, Thomas Zander, Stefan Miller
-
Publication number: 20100041038Abstract: A method for discriminating single nucleotide polymorphisms (SNPs) offers improved sensitivity and specificity. A test kit is also provided. The method may be for genotyping in typing assays applied to a biological sample. The method may include steps of performing a real-time amplification of the target, generating multiple copies of amplicons, in presence of at least two different labeled probes, each probe allowing real-time detection at the SNP position of both the wildtype and at least one possible mutation, assessing the discriminatory variable value(s) based on the signals of each combination of two detection probes, and discriminating between the genotypes. The methods may be for diagnostic, preventive and therapeutic applications.Type: ApplicationFiled: February 5, 2008Publication date: February 18, 2010Applicant: BIOMERIEUX S.A.Inventors: Jos Weusten, Maarten Johannes David De Kock
-
Patent number: 7563592Abstract: An alizarin-based chromogenic substrate, a composition containing the substrate, and its use to detect the presence of an enzymatic activity are disclosed. The substrate is particularly applicable in the field of biological diagnosis.Type: GrantFiled: March 31, 2006Date of Patent: July 21, 2009Assignee: Biomerieux, S.A.Inventors: Lyle Armstrong, Arthur James
-
Publication number: 20090178935Abstract: A method to optimize the amperometric detection in a microsystem consists in limiting the detection to times when the diffusion layer (18-20) of the analyte to detect remains smaller than the microchannel (7) height. The charge detected during the second part of the amperometric measurement (which corresponds to the integral of the measured current over the corresponding time period) can also be considered so as to remove the contribution of the capacitive current and, when applicable, of the current resulting from the reduction or oxidation of the analyte molecules present in a recess above the electrode at the beginning of the detection. A microfluidic amperometric sensor for performing the method comprises at least one microchannel (7) having at least one electrode (15-17), integrated in one wall of the microchannel, and having a characteristic length or radius which is smaller than half the microchannel height.Type: ApplicationFiled: March 29, 2007Publication date: July 16, 2009Applicants: DIAGNOSWISS S.A., BIOMERIEUX S.A.Inventors: Frederic Reymond, Joel Stephane Rossier, Patrick Morier
-
Patent number: 7537730Abstract: A reaction card (1) having a body, a front surface (62) and a rear surface (63) defined by an edge (68), at least one inlet (2, 3, 4 and/or 5) and at least one outlet (7and/or 8), connected by a network of channels (64) constituting at least one reaction path for at least one fluid which is directed via valves (11to 36 and/or 61); each valve includes a flexible film (67) which can be deformed to allow fluid passage, the film being fixed on the card's rear surface at a peripheral indentation of an assembly of channels associated with the valve; the card includes channels flush with at least one of its surfaces, the channels being of two different cross-sections, a small cross-section serving as a reaction compartment; each front or rear surface is delimited by at least one film (48, 49, 65, 66 and/or 67).Type: GrantFiled: August 27, 2001Date of Patent: May 26, 2009Assignee: Biomerieux S.A.Inventors: Bruno Colin, Cécile Paris, Bernard Limon, Patrick Broyer, Charles Rogers
-
Patent number: 7517653Abstract: This invention concerns a test method for the purposes of diagnosis, prognosis or therapeutic monitoring in male patients with adenocarcinoma of the prostate or benign prostatic hyperplasia, which does not require a biopsy of prostate tissue, and which exploits the combination of at least one tissue-specific marker and at least one marker for inflammation in order to arrive at a diagnosis. The invention also concerns a process using such a method, an immunological test to implement such a process, and a diagnostic kit. The invention is applied to the diagnosis of adenocarcinoma of the prostate and benign prostatic hyperplasia.Type: GrantFiled: June 12, 2001Date of Patent: April 14, 2009Assignee: Biomerieux S.A.Inventor: Jean-Philippe Charrier
-
Patent number: 7510843Abstract: The invention concerns the use of at least one polypeptide comprising a protein fragment to obtain a diagnostic, prognostic, prophylactic or therapeutic composition for detecting, preventing or treating a pathological condition associated with a degenerative and/or neurological and/or autoimmune disease, said protein being selected among the proteins whereof the peptide sequence in native state corresponds to SEQ ID No 1, SEQ ID No 2, SEQ ID No 3, SEQ ID No 4, SEQ ID No 5, SEQ ID No 6, SEQ ID No 7, SEQ ID No 8, SEQ ID No 9, SEQ ID No 10, SEQ ID No 11, SEQ ID No 12, SEQ ID No 13, SEQ ID No 14, SEQ ID No 15, SEQ ID No 16, SEQ ID No 17, SEQ ID No 18, SEQ ID No 19, SEQ ID No 20, SEQ ID No 21, SEQ ID No 22, SEQ ID No 23, SEQ ID No 24, SEQ ID No 25, SEQ ID No 26, SEQ ID No 27, SEQ ID No 28, and SEQ ID No 29, and the peptide sequences having at least 70% identity, preferably at least 80% identity and advantageously at least 98% identity with any one of the peptide sequences SEQ ID No 1 to SEQ ID No 8 and SEQ ID No 1Type: GrantFiled: June 12, 2006Date of Patent: March 31, 2009Assignee: Biomerieux S.A.Inventors: Dominique Roecklin, Hanno Kolbe, Marie-Helene Charles, Carine Malcus, Lyse Santoro, Herve Perron
-
Patent number: 7491497Abstract: A device for implementing a test card inside which sequential reactions and fluid transfer operations are performed under the effect of a control integrated into the card; the card includes at least two sequential reaction systems arranged in parallel, each reaction system having at least two fluid transfer systems arranged in series. A test card and the method for implementing the device and the card are also disclosed. The device contains at least one actuator per reaction line, and all the actuators are at a regular distance from one another, with the actuators preferably being equidistant. The invention is particularly applicable for the micromanipulation of fluids in biological applications.Type: GrantFiled: October 18, 2005Date of Patent: February 17, 2009Assignee: Biomerieux S.A.Inventors: Bruno Colin, Jacques Dachaud, Marie Privat, Cécile Paris
-
Publication number: 20090029360Abstract: Oligonucleotides used to prime in vitro nucleic acid amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species within the genus Mycobacterium are disclosed. Kits including such oligonucleotides are disclosed. Methods of detecting Mycobacterium species using the oligonucleotides in in vitro nucleic acid amplification are disclosed.Type: ApplicationFiled: November 12, 2007Publication date: January 29, 2009Applicants: GEN-PROBE INCORPORATED, BIOMERIEUX S.A.Inventors: Steven T. Brentano, Markus T. Jucker, Francisco D. Delgado, Philippe Cleuziat, Marc Rodrigue
-
Publication number: 20080290321Abstract: The invention relates to fluorescent polymers soluble in an aqueous solution carrying at least 5 fluorophores which are distributed on the polymer and which exhibit the following properties: the fluorophores are water-soluble, the fluorophores are not self-associated in water at a concentration of less than or equal to 10?4 mol/l, preferably at a concentration of less than or equal to 10?3 mol/l, the fluorophores which are free in the aqueous solution have a molar extinction coefficient of greater than 1000 M?1·cm?1, preferably greater than 5000 M?1·cm?1, the fluorophores which are free in the aqueous solution have a quantum yield of greater than 0.3, preferably greater than 0.6, said polymers having a fluorescence amplification factor of greater than or equal to 0.35 per kg/mol of polymer, preferably greater than 0.45 per kg/mol of polymer.Type: ApplicationFiled: June 30, 2006Publication date: November 27, 2008Applicants: BIOMERIEUX S.A., CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, INSTITUTO SUPERIOR TECNICOInventors: Marie-Therese Charreyre, Bernard Mandrand, Jose Manuel Gaspar Martinho, Paula Relogio, Jose Paulo Sequeira Farinha
-
Patent number: 7407775Abstract: A substrate which can be used for the direct identification of pathogenic bacteria belonging to the genus Listeria by detecting an esterase activity other than Phosphatidyl Inositol-specific Phospholipase C (PI-PLC), an esterase which is specific to the species Listeria monocytogenes. The use of two substrates, one as described above and the other specific for all or some members of the genus Listeria, and a reaction medium containing such a substrate or such a combination of substrates are disclosed. An identification method which exploits such culture media is also disclosed. The invention is particularly applicable in the field of diagnosis.Type: GrantFiled: August 3, 2006Date of Patent: August 5, 2008Assignee: Biomerieux S.A.Inventors: Céline Roger-Dalbert, Laurence Barbaux
-
Patent number: 7396690Abstract: A method for treating magnetic particles present in a solution in a container by redispersion, rinsing or displacement, the particles having novel characteristics under the effect of a specific magnet and being associated or not with biological entities. The magnetic particles undergo at least one low-intensity magnetization where they are disposed in filaments oriented according to the north-south axis of the magnet; the magnetization source and/or container is/are displaced while the magnetic effect is maintained on the magnetic particles; the magnetization source and/or container is/are displaced or the, magnetization is stopped, in order to suppress the magnetic field on the magnetic particles. Preferably, the invention can be used in the field of biology.Type: GrantFiled: July 9, 2002Date of Patent: July 8, 2008Assignee: BioMerieux S.A.Inventor: Bruno Colin
-
Patent number: 7374877Abstract: Sequences of nucleic acid oligonucleotides for amplifying different portions of gag and pol genes of HIV-1 and for detecting such amplified nucleic acid sequences are disclosed. Methods of amplifying and detecting HIV-1 nucleic acid in a biological sample using the amplification oligonucleotides specific for gag and pol target sequences are disclosed.Type: GrantFiled: June 3, 2005Date of Patent: May 20, 2008Assignees: Gen-Probe Incorporated, BioMerieux S.A.Inventors: Yeasing Y. Yang, Steven T. Brentano, Odile Babola, Nathalie Tran, Guy Vernet
-
Patent number: 7326532Abstract: A method of detecting rpoB sequences of Mycobacterium tuberculosis present in a biological sample that includes steps of amplifying the M. Tuberculosis rpoB sequence in vitro in a nucleic acid amplification mixture that includes specific disclosed primer sequences, and detecting the amplified sequences by using probes that provide information by their specific hybridization to portions of the amplified nucleic acid is disclosed. Compositions for amplifying and detecting in vitro the rpoB sequences of M. Tuberculosis in a sample are disclosed.Type: GrantFiled: July 28, 2006Date of Patent: February 5, 2008Assignees: Gen-Probe Incorporated, BioMerieux S.A.Inventors: Markus T. Jucker, Steven T. Brentano, Francisco D. Delgado, Philippe Cleuziat
-
Patent number: 7294489Abstract: Oligonucleotides used to prime in vitro nucleic acid amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species within the genus Mycobacterium are disclosed. Kits including such oligonucleotides are disclosed. Methods of detecting Mycobacterium species using the oligonucleotides in in vitro nucleic acid amplification are disclosed.Type: GrantFiled: September 18, 2003Date of Patent: November 13, 2007Assignees: Gen-Probe Incorporated, BioMerieux S.A.Inventors: Steven T. Brentano, Markus T. Jucker, Francisco D. Delgado, Philippe Cleuziat, Marc Rodrigue