Abstract: Disclosed is a device for separating a cellular component from a multicomponent fluid. The device can include a body, a first acoustic wave generator, and a second acoustic wave propagating component. The body can define a channel having a first surface, an opposing second surface, a first side, and an opposing second side. The channel can extend along a longitudinal axis from a first end to an opposing second end. The first acoustic wave generator can be coupled to the first surface. The second acoustic wave propagating component can be coupled to the second surface. The first acoustic wave generator and second acoustic wave propagating component can be configured to generate a bulk standing acoustic wave in the channel.

Abstract: Disclosed is a separator-concentrator, such as for separating and concentrating platelet-rich-plasma (PRP) from whole blood that is suitable for office use or emergency use for trauma victims. The PRP separator comprises a motorized centrifugal separation assembly and a concentrator assembly. The centrifugal separation assembly comprises a centrifugal drum separator and a motor having a drive axis connected to the centrifugal drum separator. The concentrator assembly comprises a water-removal module for preparing PRP concentrate.

Abstract: A plasma concentrator having a concentrator chamber, concentrator gel beads, a filter, and an agitator. The agitator end is positioned in the concentrator chamber and supported for rotation about its central axis and for reciprocal movement along its central axis. A filter is selected to block effective flow of plasma therethrough under ambient gravity conditions and permit plasma and plasma concentrate flow therethrough under centrifugal forces of the separation gravity. A method concentrates plasma by removing water without significantly denaturing the fibrinogen in the plasma. Then centrifugal force can be applied to the concentrated plasma in an amount sufficient to separate a substantial portion of the plasma concentrate from the beads.

Abstract: A device for repairing a hernia defect in a patient, the device having a biocompatible mesh material having a treated surface and a tissue composition coated on at least a portion of the treated surface, where the tissue composition originates from the patient. A method of preparing a surgical mesh for repairing a hernia defect, comprises placing a tissue specimen into a container, centrifuging the container to separate the specimen into at least two fractions, then drawing a selected fraction from the container, treating a surface of a biocompatible mesh and coating the surface-treated biocompatible mesh with the selected fraction.

Abstract: Methods for treating a cartilage defect comprising fractioning blood to produce a blood component, shaping the cartilage defect to expose subchondral bone, microfracturing the subchondral bone, and applying the blood component to the microfractured subchondral bone. The blood component can comprise platelet-poor plasma.

Abstract: Methods for promoting angiogenesis comprising administering platelet-rich plasma to a site and stimulating the site with an electromagnetic field. Platelets include platelet-rich plasma and compositions can further include stem cells such as adipose stromal cells and cells derived from bone marrow aspirate. Methods also comprise isolating platelets from a patient's blood, forming a composition including the platelets, delivering the composition to a site in need of treatment, and electrically stimulating the site.

Abstract: Methods for treating a tissue defect include applying therapeutic compositions to the tissue defect. Treatment of a tissue defect, including wound healing can be enhanced by a process including obtaining blood compatible with the subject, fractionating the blood from the subject to produce a blood component, obtaining stromal cells from the subject, combining the stromal cells and the blood component to form a therapeutic composition, and delivering the therapeutic composition to the tissue defect. Therapeutic compositions for promoting wound healing include isolated stromal cells and a blood component.

Abstract: An apparatus that allows for separating and collecting a fraction of a sample. The apparatus, when used with a centrifuge, allows for the creation of at least three fractions in the apparatus. It also provides for a new method of extracting the buffy coat phase from a whole blood sample. A buoy system that may include a first buoy portion and a second buoy member operably interconnected may be used to form at least three fractions from a sample during a substantially single centrifugation process. Therefore, the separation of various fractions may be substantially quick and efficient.