Abstract: A process to obtain multiple bacterial cellulose sheets without the need to replace new fermentation medium in the tray is provided. The medium is prepared and medium components in water are dissolved. The medium is sterilized and heated to 115° C. for 20 minutes, cooled to 30° C., inoculated with a suspension of bacteriae, and distributed in the trays that are left to rest. The sheets are harvested and drained over the corresponding trays. Further steps include repetition of the fermentation in the trays left to rest and harvest of the sheets from the trays, draining them over the corresponding trays until the volume of medium in the tray is 15%-25% of the initial volume. Then, a new medium should be added. Fermentation and draining is repeated until the volume of medium in the tray is 15%-25% of the initial volume, when new medium is added and the cycle is re-started.