Abstract: An apparatus in which cryoprotectant in cryopreserved samples is removed with the use of turbulence and convective-dispersion around the cells within the primary cryopreservation container. Convective-dispersion is applied via the combined action of gravity and pulsatile transmembrane flow of buffer through the pores of containers fabricated from appropriate membrane material. By using mechanical forces to aid the process of cryoprotectant removal, the benefits of slow cryoprotectant removal are retained while the actual removal time is markedly decreased.
Abstract: A process and apparatus in which cryoprotectant in cryopreserved samples is removed with the use of turbulence and convective-dispersion around the cells within the primary cryopreservation container. Convective-dispersion is applied via the combined action of gravity and pulsatile transmembrane flow of buffer through the pores of containers fabricated from appropriate membrane material such as that disclosed in U.S. Pat. No. 5,026,342 and U.S. Pat. No. 5,261,870, as incorporated herein by reference above. By using mechanical forces to aid the process of cryoprotectant removal, the benefits of slow cryoprotectant removal are retained while the actual removal time is markedly decreased.
Abstract: A synthetic peptide with enhanced pro-fertility action was produced by inclusion of additional amino acids at the carboxyl end of a previously disclosed synthetic peptide. Improvement in bioactivity over the previously disclosed peptide was demonstrated. A direct comparison of an earlier known synthetic peptide and an extended peptide involved brief exposure of sperm in vitro to one or the other peptide at several concentrations. When sperm then were evaluated in vitro using an egg-membrane substrate, an increased percentage of sperm bound for cells exposed to the new extended peptide. Similarly, when fertility of sperm after artificial insemination was the criterion, a greater percentage of eggs was fertilized by sperm exposed to the new extended peptide.
Type:
Grant
Filed:
March 12, 1999
Date of Patent:
August 8, 2000
Assignee:
BioPore, Inc.
Inventors:
Roy H. Hammerstedt, Surinder P. S. Gill, Rupert P. Amann
Abstract: A long rectangular slotted-sheath construct generally referred to as a "cassette," functions both in the positioning of and the treating of the individual, cell-containing primary units held therein. The invention also embraces a cooling and thawing system within which primary containers of cells, with or without a surrounding cassette, may be cooled, stored and rewarmed.
Type:
Grant
Filed:
August 19, 1998
Date of Patent:
May 23, 2000
Assignee:
BioPore, Inc.
Inventors:
Roy H. Hammerstedt, Wesley Glebe, Rupert P. Amann
Abstract: A noningestible separation barrier having one or more pores or micropores (of one or more diameters) therein, with the pores or micropores being initially plugged with one or more materials selected for its/their solubility and/or integrity characteristics relative to certain environmental conditions. Ordinarily, the pores or micropores of the noningestible separation barrier are initially filled with at least one material having greater erodibility, under a given environmental condition, than the material constituting the separation barrier itself.
Type:
Grant
Filed:
February 6, 1991
Date of Patent:
November 16, 1993
Assignee:
BioPore, Inc.
Inventors:
Roy H. Hammerstedt, Alec D. Keith, Rupert P. Amann
Abstract: A noningestible separation barrier having one or more pores of micropores (of one or more diameters) therein, with the pores or micropores being initially plugged with one or more materials selected for its/their solubility and/or integrity characteristics relative to certain environmental conditions. Ordinarily, the pores or micropores of the noningestible separation barrier are initially filled with at least one material having greater erodibility, under a given environmental condition, than the material constituting the separation barrier itself. The combination of the release rate (if any) or other membrane characteristic of the separation barrier, combined with the release rate and/or erosion life of the plugged pores, enables complex separations including variable release of cells, colloids, solutes or solvents over time, such as when the plugged pores remain intact until erosion is triggered by an environmental change such as solvent addition or ionic conditions or pH or thermal change.
Type:
Grant
Filed:
March 14, 1990
Date of Patent:
June 25, 1991
Assignee:
Biopore, Inc.
Inventors:
Roy H. Hammerstedt, Alec D. Keith, Rupert P. Amann