Abstract: A method for producing a stable polymerized hemoglobin blood-substitute from blood. The method of this invention includes contacting a polymerized hemoglobin solution with a hydroxyapatite HPLC column and recovering the substantially pure polymerized hemoglobin.

Abstract: A method is disclosed treating a tumor in a host by administering a nonemulsified ultrapurified polymerized hemoglobin solution to the host and also administering a chemotherapeutic agent to the host. In a particularly preferred embodiment, the hemoglobin is bovine hemoglobin.

Abstract: An enzymatic process for the production of 7-amino cephalosporanic acid from cephalosporine C is disclosed. The process is a two-stage enzymatic reaction which can be performed in a single reactor. The invention further includes a mutant strain of Trigonopsis variabilis which produces increased amounts of D-amino acid oxidase, and a mutant strain of Acinetobacter sp., which increased amounts of deacylase, which are enzymes necessary for the present process.

Abstract: An enzyme preparation that exhibits cephalosporin haloperoxidase activity is isolatable from a microorganism species of the Rathayibacter genus. This enzyme preparation can convert cephalexin to a halogenated cephalosporin antibiotic in a single step. A particular, unique microorganism that can provide the cephalosporin haloperoxidase enzyme preparation is Rathayibacter biopuresis.

Abstract: A method is disclosed treating a tumor in a host by administering an ultrapurified polymerized hemoglobin solution to the host and thereafter administering ionizing radiation, such as X-rays, to the tumor. In a particularly preferred embodiment, the hemoglobin is bovine hemoglobin.

Abstract: A blood substitute and plasma expander comprising a cross-linked, substantially endotoxin-free hemoglobin solution and process for preparing same. The process comprises fractionating whole blood, separating out a stromal-free, sterile hemoglobin solution, chromatographically separating endotoxins from said hemoglobin solution and crosslinking the resulting endotoxin-free hemoglobin solution.

Abstract: A valve is disclosed for allowing a sample to be withdrawn from a processing line under sterile conditions. A tip plug is disposed within a bore and is movable between a closed position and an opened position by rotation of a knob. When the tip plug is in the closed position, a sealing land at the tip plug seals a process stream from a sample port, and an annulus disposed about the tip plug provides fluid communication between a flush port and the sample port. A sterilizing fluid is directed between the flush port and the sample port through the annulus for sterilizing the sample port. The valve is opened by moving the tip plug within the bore, whereby the sealing land moves across a sample port orifice defined by the sample port to thereby provide fluid communication between the process stream and the sample port for withdrawal of a sample from the process stream under sterile conditions.

Abstract: A blood substitute and plasma expander comprising a cross-linked, substantially endotoxin-free homoglobin solution and process for preparing same. The process comprises fractionating whole blood, separating out a stromal-free, sterile hemoglobin solution, chromatographically separating endotoxins from said hemoglobin solution and crosslinking the resulting endotoxin-free hemoglobin solution.