Abstract: The L1/E1 gene region of the HPV virus maybe deleted during integration into the genome of the host cell, but the E6/E7 gene region is always retained. There is a need to detect HPV infection and cervical cancer in way that provides information about the stage of infection so that the proper treatment can be undertaken.
Abstract: High risk carcinogenic human types 16, 18 and 33 are distinguished from low risk human papillomavirus types 6 and 11 in a sample of human cervical tissue. A selected characteristic portion of the E6 region of the virus defined by specific oligonucleotide primers is amplified using a polymerase chain reaction. The presence or absence of the characteristic portion of the E6 region is detected by gel electrophoresis or using a labeled oligonucleotide probe.
Abstract: Carcinogenic human papillomavirus types HPV16 and HPV18 are detected in a sample of cervical tissue. A selected characteristic DNA portion of the virus defined by oligonucleotide primers is amplified using a polymerase chain reaction involving successive heating and cooling steps, for example up to 250,000 copies. The presence/absence of the characteristic cloned DNA portion is detected by gel electrophoresis or using a labelled oligonucleotide probe.