Abstract: The luminescence imaging apparatus comprises: a stage receiving a sample (2) emitting luminescence information about the sample; a light source (8) illuminating the stage; and an electronic control unit defining time frames; a combined light signal corresponding to a combination of luminescence information and of the reflection on the sample of the illumination. During each time frame, the detection apparatus (9) acquires both first data relating to the luminescence information, and also second data relating to the second light signal.