Abstract: Provided is a method for obtaining high-yield, stable expression cell clones from myeloma cell lines in a protein-free culture medium. The method is used for industrial production of a recombinant antibody, and includes three stage: (1) adapting to a protein-free culture medium, statically culturing cells at a low density, and gradually reducing a fat-rich supplement to a chemical culture medium; (2) adapting to a protein-free culture medium; culturing cells at a high density, and using a perfusion fermentation system in a laboratory scale; and (3) screening high-yield, stable expression cell clones from the cells after fermentation ends. The cell clone may be used to produce a humanized anti-NeuGcGM3 14F7 recombinant antibody.

Abstract: A process of preparing a burdock composite micron essence at least includes the following. Dissolved and heated phospholipid is added to a vegetable oil extract and then stirred. After stirring, the above mixture is pour into a heated glycerol and then further stirred and a burdock extract aqueous solution is added. The aforementioned mixed solution is stirred at high speed, the emulsion-homogenized solution is subject to high-pressure homogenization, and a filtration membrane is used to filter the homogenized solution obtained to get a purple transparent burdock concentrate. The burdock concentrate is used to prepare the formulation of nano-micro emulsion which is easily absorbed by human body and thus grants improved health benefits.

Abstract: A process of preparing a burdock composite micron essence at least includes the following. Dissolved and heated phospholipid is added to a vegetable oil extract and then stirred. After stirring, the above mixture is pour into a heated glycerol and then further stirred and a burdock extract aqueous solution is added. The aforementioned mixed solution is stirred at high speed, the emulsion-homogenized solution is subject to high-pressure homogenization, and a filtration membrane is used to filter the homogenized solution obtained to get a purple transparent burdock concentrate. The burdock concentrate is used to prepare the formulation of nano-micro emulsion which is easily absorbed by human body and thus grants improved health benefits.