Abstract: Invention is related to novel compounds -5-aryl-4-(5-substituted 2,4-dihydroxyphenyl)-1,2,3-thiadiazoles with general formula (I). The compounds can be used in biomedicine as active ingredients in pharmaceutical formulations, because they inhibit Hsp90 chaperone which participate in cancer progression. This invention is also related to new intermediate compounds which are used for the synthesis of thiadiazoles of general formula (I).
Type:
Grant
Filed:
June 18, 2008
Date of Patent:
November 20, 2012
Assignee:
Biotechnologijos Institutas
Inventors:
Daumantas Matulis, Inga Cikotiene, Egidijus Kazlauskas, Jurgita Matuliene
Abstract: The present invention relates to a use of non-cofactor compounds, represented by formulas (I) or (II) wherein R and Z are independently selected from H, D, C1-C12-alkyl, preferably C1-C4-alkyl, alkenyl, alkinyl, phenyl or -LX, wherein X represents a functional group or a reporter group attached via a linker group L, and QH is selected from —SH, —SeH, —NHNH2 or —ONH2, for a targeted modification or derivatization of a biomolecule by covalent coupling to the biomolecule in the presence of a directing methyltransferase. Further development of the method of targeted modification and derivatization are the method for targeted labeling a biomolecule and method for detecting unmethylated target sites in a biomolecule comprising modification of the biomolecule according to the present invention.
Type:
Application
Filed:
April 1, 2010
Publication date:
April 19, 2012
Applicant:
BIOTECHNOLOGIJOS INSTITUTAS
Inventors:
Saulius Klimasauskas, Zita Liutkeviciute, Edita Kriukiene
Abstract: The present invention relates to targeted conversion of alpha-hydroxyalkylated residues in biomolecules in the presence of a directing methyltransferase, namely to targeted removal of the alpha-hydroxyalkyl moieties to give unmodified residues, or targeted derivatization of the alpha-hydroxyalkyl groups by covalent coupling of non-cofactor compounds represented by formula HQ-LX1 wherein X represents a functional group or a reporter group attached via a linker moiety L, and QH is selected from HS—, HSe—, HO—H2N—, HN3 or HCN in the presence of a directing methyltransferase. Further development of the method of targeted conversion comprises methods for targeted labeling a biomolecule and method for detecting hydroxymethylated target sites in a biomolecule according to the present invention.
Type:
Application
Filed:
April 1, 2010
Publication date:
April 12, 2012
Applicant:
BIOTECHNOLOGIJOS INSTITUTAS
Inventors:
Saulius Klimasauskas, Zita Liutkeviciute, Edita Kriukiene
Abstract: Provided is a use of a recombinant chimaeric protein as an immunogen in a process for producing a monoclonal antibody, wherein the recombinant chimaeric protein is assembled into a virus-like particles, and includes a foreign protein or peptide or a fragment thereof.
Type:
Grant
Filed:
October 12, 2007
Date of Patent:
April 5, 2011
Assignee:
Biotechnologijos Institutas
Inventors:
Aurelija Zvirbliene, Alma Gedvilaite, Rainer Ulrich, Kestutis Sasnauskas