Abstract: A method is presented for catalyzing the conversion of substrate into product in an organic reaction solvent with an enzyme-surfactant ion pair. The enzyme-surfactant ion pair comprises the enzyme catalyzing the reaction and a surfactant capable of forming an ion pair with the enzyme. Water present in the organic solvent at a concentration of about 0.03% to about 2.5% is sufficient to enhance the rate of catalysis and stabilize the enzyme without substantially increasing the rate of hydrolysis when compared to the anhydrous enzyme.
Type:
Grant
Filed:
October 16, 1997
Date of Patent:
January 9, 2001
Assignees:
BioTechnology Research & Develop. Corp., The University of Iowa Research Foundation
Inventors:
Jonathan S. Dordick, Vikram M. Paradkar, Maria V. Sergeeva