Abstract: The present invention provides non-invasive methods and compositions to differentiate, with a high level of sensitivity and specificity, swine that are genetically susceptible to diseases associated with F18 E. coli infection, from resistant swine. DNA polymorphisms in the swine alpha (1,2) fucosyltransferase 1 (FUT1) gene were used to differentiate resistant from susceptible swine. The invention includes a polypeptide with amino acid substitutions, encoded by the nucleotide polymorphisms, a molecular diagnostic assay, and a kit for the differentiation, of E. coli F18-adhesion resistant, heterozygous (carrier) and homozygous susceptible pigs. The molecular test identifies susceptibility to oedema disease and postweaning diarrhea with high sensitivity and specificity, therefore, is useful to swine breeder in their effort to enhance for resistance. Information on the polymorphisms of the present invention provides insight into causation and treatment of E. coli associated intestinal disorders.
Type:
Grant
Filed:
November 19, 1999
Date of Patent:
July 22, 2003
Assignees:
Biotechnology Research & Development Corp., The United States of America as represented by the Secretary
of Agriculture, Swiss Federal Institute of Technology
Abstract: A method of preparing a polymer-protein composite based upon placing a protein in solution in an organic phase via the ion-pairing of the protein with a surfactant. The polymer-protein composites are useful, for example, as highly active and stable catalysts, in for example, paints and coatings, as well as in medical application.
Type:
Grant
Filed:
November 10, 1999
Date of Patent:
September 18, 2001
Assignee:
Biotechnology Research & Development Corp.
Inventors:
Jonathan S. Dordick, Ping Wang, Maria Vladimir Sergeeva, Scott Joel Novick
Abstract: Articles are provided in which a self-supporting structure formed of natural polymer has a self-adherent, moisture resistant hydroxy-functional polyester on the structure surface. The self-supporting structure preferably is a starch and polyvinyl alcohol blend in an expanded form. The articles typically do not delaminate even when soaked in water, and are biodegradable.
Type:
Grant
Filed:
January 15, 1999
Date of Patent:
March 21, 2000
Assignees:
The United States of America as represented by the Secretary of Agriculture, Biotechnology Research & Development Corp.
Inventors:
William M. Doane, John W. Lawton, Jr., Randal Shogren
Abstract: Compositions of the invention include particular hydroxy-functional polyesters and a natural polymer. Among articles that can be made from such compositions are those with sufficiently strong tensile strengths as to form disposable utensils. However, the compositions are more environmentally friendly than commodity plastics such as polyethylene or polystyrene. Starches are particularly preferred as the natural polymers for inclusion in the compositions.
Type:
Grant
Filed:
September 8, 1997
Date of Patent:
February 15, 2000
Assignees:
Biotechnology Research & Development Corp., The United States of America as represented by the Secretary of the Agriculture
Inventors:
Julious L. Willett, William M. Doane, Wayne Xu, Michael N. Mang, Jerry E. White
Abstract: A method of preparing a polymer-protein composite including polymerizing a monomer in the presence of a protein dissolved in an organic phase via the ion-pairing of the protein with a surfactant. The polymer-protein composites are useful, for example, as highly active and stable catalysts, in for example, paints and coatings, as well as in medical application.
Type:
Grant
Filed:
October 9, 1997
Date of Patent:
June 22, 1999
Assignee:
Biotechnology Research & Development Corp.
Inventors:
Jonathan S. Dordick, Ping Wang, Maria Vladimir Sergeeva, Scott Joel Novick
Abstract: Articles are provided in which a self-supporting structure formed of natural polymer has a self-adherent, moisture resistant hydroxy-functional polyester on the structure surface. The self-supporting structure preferably is a starch and polyvinyl alcohol blend in an expanded form. The articles typically do not delaminate even when soaked in water, and are biodegradable.
Type:
Grant
Filed:
June 28, 1996
Date of Patent:
January 19, 1999
Assignees:
The United States of America as represented by the Secretary of Agriculture, Biotechnology Research & Development Corp.
Inventors:
William M. Doane, John W. Lawton, Jr., Randal Shogren
Abstract: Organic enzyme solutions comprise an ion pair complex of an enzyme and a surfactant, and an organic solvent in which the enzyme-surfactant ion pair is dissolved. The solution has catalytic activity at least an order of magnitude greater than a suspension of an equal amount of the enzyme and organic solvent without ion pair complexes. The enzyme is preferably a hydrolase with an acyl transferase activity and the surfactant is Aerosol OT (sodium bis(2-ethylhexyl) sulfosuccinate) at low concentrations.
Type:
Grant
Filed:
June 1, 1995
Date of Patent:
February 17, 1998
Assignees:
University of Iowa Research Foundation, Biotechnology Research & Development Corp.
Inventors:
Jonathan S. Dordick, Vikram M. Paradkar