Patents Assigned to Bioteknologisk Institut
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Patent number: 6924366Abstract: A method of producing hexose oxidase by recombinant DNA technology, recombinant hexose oxidase and the use of such enzyme, in particular in the manufacturing of food products such as doughs and dairy products, animal feed, pharmaceuticals, cosmetics, dental care products and in the manufacturing of lactones. Suitable sources of DNA coding for the enzyme are marine algal species including Chondrus crispus, Iridophycus flaccidum and Euthora cristata. In useful embodiments, the recombinant hexose oxidase is produced by Pichia pastoris, Saccharomyces cerevisiae or E. coli.Type: GrantFiled: April 3, 2001Date of Patent: August 2, 2005Assignee: Bioteknologisk InstitutInventors: Peter Stougaard, Ole Cai Hansen
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Publication number: 20040038263Abstract: A method of identifying nucleotide sequences coding for signal peptides in lactic acid bacteria, using a DNA molecule comprising a transposon including a promoterless reporter gene from which DNA molecule a region between the LR and the reporter gene is deleted and the DNA molecule comprises a DNA sequence coding for a secretion reporter molecule. By deleting the region between the LR and the reporter gene, stop codons in-frame with the secretion reporter molecule is removed which upon transposition permits translational fusions from upstream the LR.Type: ApplicationFiled: May 8, 2003Publication date: February 26, 2004Applicant: Bioteknologisk InstitutInventors: Peter Ravn, Soeren Michael Madsen, Astrid Vrang, Hans Israelsen, Mads Groenvold Johnsen, Lars Bredmose, Jose Arnau
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Publication number: 20030134353Abstract: It is an object of the present invention to provide fungal host organisms capable of expressing recombinant proteins while at the same time exhibiting satisfactory growth characteristics. It is a further object to provide—in a single fungal host organism—the characteristic of homogeneous growth and low viscosity typically associated with yeast organisms, and the capability for high protein secretion normally associated with filamentous fungi. It is a yet further object of the invention to provide useful tools for genetic analysis in zygomycetes, including dimorphic zygomycetes. Accordingly, the present invention relates to a recombinant, fungal cell or dimorphic fungal cell comprising a regulatable expression of a regulator of morphology. The nucleotide sequence encoding the at least one regulator of morphology is operably linked to an expression signal not natively associated therewith.Type: ApplicationFiled: March 8, 2002Publication date: July 17, 2003Applicant: Bioteknologisk InstitutInventors: Anne Mette Wolff, Karen Fuglede Appel, Jesper Breum Petersen, Ulla Poulsen, Jose Arnau, Mette Dorph Jacobsen
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Publication number: 20020106725Abstract: A method of producing hexose oxidase by recombinant DNA technology, recombinant hexose oxidase and the use of such enzyme, in particular in the manufacturing of food products such as doughs and dairy products, animal feed, pharmaceuticals, cosmetics, dental care products and in the manufacturing of lactones. Suitable sources of DNA coding for the enzyme are marine algal species including Chondrus crispus, Iridophycus flaccidum and Euthora cristata. In useful embodiments, the recombinant hexose oxidase is produced by Pichia pastoris, Saccharomyces cerevisiae or E. coli.Type: ApplicationFiled: April 3, 2001Publication date: August 8, 2002Applicant: BIOTEKNOLOGISK INSTITUTInventors: Peter Stougaard, Ole Cai Hansen
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Patent number: 6251626Abstract: A method of producing hexose oxidase by recombinant DNA technology, recombinant hexose oxidase and the use of such enzyme, in particular in the manufacturing of food products such as doughs and dairy products, animal feed, pharmaceuticals, cosmetics, dental care products and in the manufacturing of lactones. Suitable sources of DNA coding for the enzyme are marine algal species including Chondrus crispus, Iridophycus flaccidum and Euthora cristata. In useful embodiments, the recombinant hexose oxidase is produced by Pichia pastoris, Saccharomyces cerevisiae or E. coli.Type: GrantFiled: September 11, 1996Date of Patent: June 26, 2001Assignee: Bioteknologisk InstitutInventors: Peter Stougaard, Ole Cai Hansen
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Patent number: 6133023Abstract: Expression vectors capable of being replicated in lactic acid bacterial cells comprising a promoter region comprising (a) a promoter sequence element the function of which is regulatable by an environmental or growth condition factor and (b) at least one further nucleotide sequence element, the position, orientation, presence and/or sequence of which element has a regulatory effect on the expression of a gene operably linked to the promoter region in which vectors the position, orientation, presence and/or sequence of at least one of said elements (a) or (b) is modified relative to the position, orientation, presence and/or sequence of the corresponding non-modified element whereby the expression of the gene is altered, and a lactic acid bacterium which is transformed with such a vector as defined above are provided.Type: GrantFiled: December 29, 1997Date of Patent: October 17, 2000Assignee: Bioteknologisk InstitutInventors: Soeren Michael Madsen, Astrid Vrang, Jose Arnau, Peter Ravn, Mads Groenvald Johnsen, Hans Israelsen
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Patent number: 6004747Abstract: The invention provides nucleic acid molecules for the detection and identification of Salmonella species, methods for detecting one or more Salmonella serotpes using the nucleic acid molecules of the invention as probes or primers in DNA-based detection systems and kits for carrying out the invention.Type: GrantFiled: March 11, 1996Date of Patent: December 21, 1999Assignees: John Elmerdahl Olsen, Bioteknologisk Institut, Soren AaboInventors: John Elmerdahl Olsen, Soren Aabo, Lone Rossen, Ole Feldballe Rasmussen
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Patent number: 5837509Abstract: A method of isolating a lactic acid bacterial DNA fragment comprising a promoter, the method comprising introducing a DNA molecule comprising a transposable element comprising a promoterless structural gene as a promoter probe gene into a population of a lactic acid bacterium, methods of constructing a recombinant lactic acid bacterium comprising a regulatable promoter by using the above method, a recombinant lactic acid bacterium comprising a gene coding for a desired gene product and operably linked thereto a regulatable lactic acid bacterial promoter not natively associated with the gene, the use of such a recombinant lactic acid bacterium and recombinant plasmids comprising a regulatable lactic acid bacterial promoter.Type: GrantFiled: March 1, 1995Date of Patent: November 17, 1998Assignees: Bioteknologisk Institut, CHR Hansen's Laboratorium Danmark A/SInventors: Hans Israelsen, Egon Bech Hansen, Eric Johansen, Soeren Michael Madsen, Dan Nilsson, Astrid Vrang