Abstract: This invention relates to a process for the production of a von Willebrand factor preparation, hydroxylapatite being used as a chromatography medium.

Abstract: The invention relates to a method for separating fibronectin from plasma fractions by adjusting a pH value of less than 5.4 such that fibronectin is precipitated and extracted from the solution.

Abstract: A perfusion liquid is suctioned from a perfusion liquid reservoir (16) through the automatic two-way valve (4) by drawing a syringe (5) serving as the perfusion liquid displacement device. For this purpose, the connection from the syringe (5) to the cannula (14) is closed by the two-way valve (4). The two-way valve (4) closes the connection to the perfusion liquid reservoir due to the pressure increase during the insertion of the syringe plunger and opens the connection from the syringe (5) to the cannula (14). A pressure relief valve (7) is attached at the T piece (6), exposing a discharge line (8) in case a threshold pressure is exceeded. A check valve (11) is attached at the extension of the cannula (14), preventing the backflow from the cannula (14) into the remaining system.

Abstract: This invention relates to a process for the production of a von Willebrand factor preparation having a high specific activity, hydroxylapatite being used as a chromatography medium.

Abstract: The particle counter is equipped with a scroll pump adapted to create a gas flow through the measurement chamber with a maximum flow rate of at least 50 liters per minute. Light scattered from particles entrained in the gas flow is collected by an optical system. The product of the smallest diameter of the minimum aperture and the magnification amounts to at least 2 mm. Especially preferred is a minimum aperture with a smallest diameter of at least 2.5 mm and a magnification of at least 1.5. The optical system images a projection of the measurement volume created by intersection of the gas flow and the beam of light onto the active area of the sensor module. The sensor module 13 contains a photomultiplier-tube (PMT). Both sensitivity and accuracy of the particle counter are improved using a larger aperture and greater magnification, which is made possible by use of the photomultiplier tube.

Abstract: A humanized antibody derived from mouse monoclonal anti-CD4 antibody B-F5 is able to activate CD25+CD4+ regulatory T cells and is useful for preparing immunosuppressive compositions.

Abstract: The invention relates to a method and an apparatus for the endothelium-preserving treatment of isolated hollow organs, especially biological vessels such as blood vessels and lymphatic vessels by means of endothelium-protective perfusates or incubation solutions containing at least 0.1 percent by weight of native albumin and a nutrient substrate, preferably L-glutamine. Also disclosed are the use of the endothelium-protective perfusates for preparing hollow organs or biological vessels as a transplant for the treatment of organ diseases or vascular diseases, the use thereof for repairing endothelial lesions in isolated hollow organs and/or biological vessels, and the use thereof for preserving organs and/or vessels.

Abstract: Immunoconjugates comprising a targeting agent selectively targeting cell-surface expressed syndecan-1 and at least one effector molecule as well as in vitro and in vivo methods of using those immunocomjugates are disclosed. The effector molecule may have, in its native form, high non-selective cytotoxicity, but substantially no non-selective cytotoxicity when part of said immunoconjugate. Targeting agents include the antibody B-B4 as well as other agents that bind cell-surface expressed syndecan-1.

Abstract: Gamma sterilizable culture medium for the selectively identification of yeasts and fungi with an addition of ciprofloxacin and, if necessary streptomycin or other antibiotics.

Abstract: A gamma-sterilisable nutrient medium based on casein soya peptone agar for the detection of microorganisms in hydrogen peroxide-bearing air or on hydrogen peroxide-bearing surfaces, with a content of between 2 and 10% by weight of sodium thioglycolate, between 5 and 20% by weight of sodium thiosulfate and between 10 and 30% by weight of sodium disulfite in each case with respect to the agar. Preferably the agar used is microbial content test agar and the nutrient medium may contain between 0.1 and 0.25% by weight of sodium pyruvate with respect to the agar. If bromocresol purple and bromocresol violet are used as pH-indicators the nutrient medium may also contain polyvinylpyrrolidone.

Abstract: The present invention relates to a method for improving the growth and detection of bacteria, yeasts, fungi or cocci, by adding sterile-filtered yeast extract and/or p-iodonitrotetrazolium violet to the culture medium.

Abstract: The invention relates to polypeptides of the Kaposi sarcoma-associated herpes virus which have a part-sequence of at least 10 consecutive amino acids from the sequence Met Ser Ser Thr Gln Ile Arg Thr Glu Ile Pro Val Ala Leu Leu Ile Leu Cys Leu Cys Leu Val Ala Cys His Ala Asn Cys Pro Thr Tyr Arg Ser His Leu Gly Phe Trp Gln Glu Gly Trp Ser Gly Gln Val Tyr Gln Asp Trp Leu Gly Arg Met Asn Cys Ser Tyr Glu Asn Met Thr Ala Leu Glu Ala Val Ser Leu Asn Gly Thr Arg Leu Ala Ala Gly Ser Pro Ser Ser Glu Tyr Pro Asn Val Ser Val Ser Val Glu Asp Thr Ser Ala Ser Gly Ser Gly Glu Asp Ala Ile Asp Glu Ser Gly Ser Gly Glu Glu Glu Arg Pro Val Thr Ser His Val Thr Phe Met Thr Gln Ser Val Gln Ala Thr Thr Glu Leu Thr Asp Ala Leu Ile Ser Ala Phe Ser Gly Val Leu His Val Ser Thr Val Ile Pro Arg Asn Trp Val Asn Arg Arg Cys Val Gly Ile Lys Arg Asn Leu Thr Phe

Abstract: The present invention relates to a method for improving the growth and detection of bacteria, yeasts, fungi or cocci, by adding sterile-filtered yeast extract and/or p-iodonitrotetrazolium violet to the culture medium.

Abstract: Diagnostically relevant polypeptides and fusion proteins comprising an amino acid sequence which originates from cytomegalovirus and corresponds to a region of the major DNA-binding protein or of the C-terminal region of the tegument protein pp150 fused with at least one further fragment from another antigenic protein of cytomegalovirus are disclosed. The major DNA-binding protein is encoded by the reading frame UL57. The poly-peptides and fusion proteins according to the invention can be used in an advantageous manner in diagnostic tests and methods for the detection of IgM antibodies against cytomegalovirus.

Abstract: The invention concerns a method of detecting proteins, especially HIV and CMV antibodies in body fluids using solid-phase immune reaction with a buffer containing an animal serum and/or a non-ionic surfactant in a phosphate or carbonate buffer together with further additives selected from inorganic salts, water-soluble polymers, organic compounds, and propionic acid and their derivatives and mixtures. The buffer makes it possible to reliably eliminate false positive results.

Abstract: A method of, and support and kit for, determining blood groups by the solid-phase procedure with blood-group specific immunoglobulin-M antibodies. The support is prepared by applying to its surface a solution of the immunoglobulin-M antibodies and simultaneously applying to the surface of the support a suspension of erythrocytes that is being tested, or drying the surface of the support to form a ready-to-use reagent, and subsequently contacting the surface with a suspension of erythrocytes, and determining the blood group just after the contact occurs, the concentration of antibodies being just sufficient to create an irreversible or immobilized coating of antibody molecules on the surface that can specifically attach erythrocytes but insufficient to induce an agglutination reaction.