Abstract: Crosslinked hemoglobin preparations of extended shelf life and high oxygen transport capacity can be made by reducing a hemoglobin solution with an oxygen consuming reducing agent down to an oxygen partial pressure of 0 mbar, then adding an effector, crosslinking with a dialdehyde, reducing with a carbonyl-group-specific reducing agent, circulating through an ultrafilter, and stabilizing the product by adding a reducing agent thereto.

Abstract: A microtiter plate for blood typing consisting of a flat-bottomed plate of rigid transparent polystyrene having a plurality of wells, the polystyrene being surface treated by a corona and/or plasma method of applying high power electric energy at high frequency resulting in a permanently measurable negative ion-charge at the surface of the wells, and layers of substantially pure antiserums dried on the wells without any binder from a suspension or solution of the antiserums and tightly but releasably bound to the polystryene.

Abstract: The use of pterins to increase the activity of lymphokines and other cell growth factors, and a diagnostic or theraupeutic preparation that contains pterins combined with lymphokines. Adding pterins to lymphokines can increase the activity of the lymphokines by 3 to 5 times.

Abstract: Monoclonal antibodies that recognize a structure common to human interleukin-2 and to the light .lambda. chain of human immunoglobulin and lines of hybridoma cells that produce these monoclonal antibodies can be prepared by immunizing animals, especially mice, with human interleukin-2 (TCGF) and fusing the splenocytes obtained from the animals with animal, expecially mouse, myeloma cells to create a hybridoma. The hybridomas are raised as clones and the antibodies obtained from the individual clones tested for their specificity to human interleukin-2 (TCGF). Clones that produce antibodies with a specificity to human interleukin-2 (TCGF) are selected for further raising to prepare the antibody. The antibody is harvested from the culture medium or from the ascitic fluids of the animal, especially the mouse, with the hybridoma.

Abstract: A blood-culture flask with an integrated subculture. It comprises a flask proper that can be filled with a liquid nutrient solution and has a neck with an outside thread, a support that can be coated with a solid culture medium, a resilient stopper with an edge that projects at one end, and a screw cap. The screw cap consists of a bottom that is open above and below and has an inside thread and of a top that is open below. The inside thread is dimensioned so that it can be screwed down over the outside thread on the neck of the flask so that the upper edge of the thread is below the upper edge of the neck when screwed on. The resilient stopper is positioned inside the bottom of the screw cap so that the bottom surface of its resilient projecting edge comes to rest against the offset above the thread when not screwed on and its lower section is dimensioned so that it can be introduced tight into the neck of the flask.

Abstract: In a device for testing air for microorganism content with a rapidly rotating impeller inside a cylinder that has a backing sheet coated with culture medium against its inside surface, microorganisms that vary more extensively in size can be detected with almost equal effectiveness, the throughput of air can easily be determined, and the precipitation effect can be precisely tested because the backing sheet rotates along with the impeller, the blades of the propeller are at a low angle to the plane it rotates in, the air enters at a location that is separate from the location at which it leaves, through inlets from above and down and out through outlets, and the inner surface of the rotor below the impeller chamber is in the form of a channeling cylinder.

Abstract: An aqueous solution for suspending and storing cells, especially erythrocytes, and containing sodium chloride, glucose or fructose, adenine, and a sugar alcohol, the sugar alcohol being sorbitol or xylitol is employed to further improve the storage time and hence the survival rate of the cells and even reduce the hemolysis rate of erythrocytes in packed red blood cells.

Abstract: Crude interleukin-2 extract is subjected to group-selective dye-ligand absorption chromatography in one or more stages of purification with a matrix-gel medium consisting of a Blue A ligand or variant thereof or of a Green A ligand in a concentration of approximately 1.5 to 3.0 mg/ml of expanded matrix at a pH of approximately 6.8 to 8.5, a temperature of approximately 4.degree. to 40.degree. C., and a flowthrough rate of approximately 10 to 100 ml/h, employing an eluent. Either PHA-free or extremely pure interleukin-2 is obtained, depending on the overall number of purification stages.

Abstract: A coagulant plasma-protein solution obtained by adsorbing stabilized human plasma at least once with 20-40 mg of colloidal silicic acid per gram of plasma protein and separating the adsorbent from the adsorbate, a method of manufacturing the solution, and a pharmaceutical preparation containing the solution and intended for the intravenous treatment of complex disorders of the hemostatic system. The solution is low in fibrinogen and factors XI and XII but otherwise close in composition to the starting material.

Abstract: A bag suitable for the storage, deep-freezing and transfusion of blood and blood components formed of a calendered or extruded foil of plasticizer-free polyurethane of a thickness of about 0.1-0.5 mm, a Shore hardness A of about 60-85, temperature resistance up to about 160.degree. C. and down to about -196.degree. C. without change of properties. The bag can be used in combination with fittings and tubings of the same material to provide an overall plasticizer-free polyurethane system. The bag can be sterilized, is capable of low temperature storage, and exhibits other desirable properties without affecting the quality of its contents.

Abstract: A microfiltration device for the filtration of coagula and microaggregates from blood and blood components. The device contains a filter chamber with attaching nozzle, a filter cone containing truncoconical filter elements arranged in cascade with diminishing pore size and diminishing surface area, a drip chamber and a tailpiece. The filter chamber and filter cones have different tapers and are at a specific distance apart, the taper of the filter cone from inlet to outlet being greater than the taper of the filter chamber. The filter cone has up to four filter elements, and a spacer which extends into the filter element of smallest pore size and surface area and supports same. Each of the filter elements has at least two opposed ribs running from the base to the apex of the truncated cone. The spacer is constructed such that it serves simultaneously for the support of the ribs and for the support of the filter fabric of the smallest filter element. The device has a capacity of less than 100 ml.

Abstract: A process for obtaining hepatitis-safe, sterile, pyrogen- and stroma-free hemoglobin solutions of low potassium content comprising stirring an erythrocyte concentrate with about 1 to 6-times its volume of an about 5 to 15% aqueous solution of a sugar, sugar alcohol or high-molecular-weight colloid, adjusting the pH of the resulting suspension to about 5 to 6.5 with an acid which is physiologically tolerable as salt, allowing the erythrocytes to settle, removing the supernatant from the sediments, adjusting the pH of the sediment to about 7 to 8, homogenizing the sediment, adjusting its hematocrit to about 55 to 60%, treating the mass with a dilute beta-propiolactone solution, hemolyzing the residual solids and forming a suspension thereof, contacting the suspension with a cation exchanger in H.sup.+ form until the pH has decreased to about 5 to 5.5 thereby to precipitate the stroma, separating from the liquid the cation exchanger and precipitated stroma, and adjusting the pH to about 7.2 to 7.6.

Abstract: In a blood bag assembly comprising a blood bag and means for connecting the interior of the bag with the interior of a second bag, the improvement wherein said connecting means comprises a coupling composed of a connecting portion, a break-off portion, and a break-off point, the coupling being joined directly at the upper edge of the bag and terminates substantially evenly therewith, the break-off portion will be inside of the bag after it is broken off.

Abstract: A nutrient medium carrier system comprising a sterilizable plastic nutrient medium carrier, a sterilizable plastic cover, and a sealing foil, the nutrient medium carrier having a depression to receive the nutrient medium, a surrounding beaded edge above the surface of the nutrient medium, a handle on one end, and a flat edge running around both sides and the other end, the depression being subdivided into compartments by means of ridges; the cover having a depression and a surrounding flat edge to receive the sealing foil, the cover depression gradually widening on two sides and one end to a flat edge up to the dimensions of the nutrient medium carrier, the narrow part being of such size that both side walls and one end touch the beaded edge on two sides and on the end opposite the handle, the cover having a steplike offset with locking flanges on its upper edge to hold the nutrient medium carrier, the flat edge of the carrier contacting the steplike offset of the cover, the cover on the side on which the han

Abstract: A process for the cold sterilization of a preparation containing blood coagulation factor VIII, comprising(A) contacting a protein solution containing factor VIII with a physiologically compatible, non-ionogenic tenside, and then subjecting the solution to(B) irradiation with ultraviolet light,(C) treatment with beta-propiolactione, and(D) to an adsorption treatment with colloidal silica, steps B-C-D being performed in the sequence B-C-D, B-D-C, D-C-B or D-B-C.

Abstract: Hemoglobin molecules are coupled so as to increase their intravascular residence times without significantly diminishing their oxygen transport ability. This is achieved by coupling hemoglobin molecules to one another and/or to serum proteins and gelatin derivatives using dialdehydes such as aliphatic dialdehydes of 3 to 8 carbon atoms, optionally followed by addition of pyridoxal phosphate. The desired material isolated by ammonium sulfate precipitation, the sulfate being added before, simultaneously with, or after the dialdehyde. Solution of the precipitate followed by dialysis and/or ion exchange can also be undertaken.

Abstract: A process for the preparation of an immunoglobulin solution containing IgM in concentrated form and suited for intravenous administration, comprising treating an IgM-containing protein fraction obtained by conventional fractionation from blood plasma or serum with .beta.-propiolactone in an amount such that the ratio of .beta.-propiolactone to a 5% solution of the IgM-containing proteins is from about 0.05 to 0.15 ml per 100 ml. Advantageously, prior to the treatment with .beta.-propiolactone the IgM-containing protein fraction is freed of lipids by treatment with colloidal silica gel and with crosslinked dextrans or diethylaminoethyl cellulose. The starting material used is a Cohn fraction III of human blood plasma which has been dissolved in physiological saline solution to a concentration of about 5% protein, the treatment with .beta.-propiolactone is carried out at about 20.degree. to 37.degree. C.

Abstract: The present invention relates to a method for making fibrinogen, a prothrombin complex containing the coagulation factors II, VII, IX and X that can contain antithrombin III, antithrombin III and a solution of stable serum proteins from a blood plasma stabilized with citrate which is characterized in that from the plasma, by adsorption on colloidal silica of a specific surface of 50 to 400 m.sup.

Abstract: A culture cup for sampling and microbial-count determination, comprising a body and a tightly closing removable lid, the bottom of the body being provided with depressions for accomodation of at least one culture medium. The lid on its underside may be provided with a recess for accommodation of a condensate absorbent, and the body of the cup with a shoulder to serve as a seat for the bottom of the lid. The lid and body are threaded for engagement. By placing a culture medium in the depression, a biological specimen such as urine or blood can be added in just sufficient quantity to wet the culture so pouring and handling are reduced. Culture and analysis can then be undertaken in the usual manner using the cup body as the carrier of the culture medium.

Abstract: Antigens and their antibodies are measured in a combined radioimmunoassay by incubating a sample of an unknown fluid with a known quantity of an antibody, contacting the mixture obtained with the walls of a vessel having a coating of known antigen content, again incubating, then aspirating the mixture and washing the walls of the vessel, adding a pure radioactive tracer antigen, aspirating and washing again, and finally measuring the radioactivty of the coated parts conventionally, e.g., in a gamma counter known in the art.