Abstract: In vitro evaluation of a potentially allergenic or tissue irritating substance is conducted by cultivating test cells in the presence of the substance and the presence of up regulated genes chosen from G1P2, OASL, IFIT1, TRIM22, IFI44L, MXI, RSAD2, IFIT3, IFITM1, IFIT2, SPR, GNB2, XK, IFITM3, C 33.28 HERV-H protein mRNA, GPR15, MT1G, MT1B, MT1A, ADFP, IL8, MT1E, MT1F, MT1H, SLC30A1, SERPINB2, CD83, and TncRNA, or expression products from them, are measured. The expression products from one or more of the genes may be used for in vitro analysis of allergy or tissue irritation. A probe comprising at least three nucleic acids, preferably 3-40, especially 5-15, chosen from RNA complementary to the RNA corresponding to any of the genes may be used for in vitro analysis of allergy or tissue irritation. Further, a reagent kit comprising one or more probes that recognize products produced during the expression of any the genes is disclosed.

Abstract: The invention relates to a process for in vitro prediction of a potentially allergenic substance wherein monocytes and/or macrophages and/or myelomonocytic cell lines are cultivated in the presence of the substance and interferon-?, whereby productions of cytokines and/or neopterin are increased and measured. The allergic reaction may be estimated by measuring up regulated or down-regulated genes chosen from G1P2, OASL, IFIT1, TRIM22, IFI44L, MXI, RSAD2, IFIT3, IFITM1, IFIT2, C 33.28 HERV-H protein mRNA, IFITM3, XK, GPR15, MT1G, MT1B;MT1A, ADFP, IL8, MT1E, MT1F, MT1H, SLC30A1, SERPINB2, CD83, TncRNA or expression products from them are measured. The invention also relates to a reagent kit for use in the process comprising interferon-? and reagents which recognise cytokines preferably each of IL-8 and IL-10, and neopterin and /or reagents such as probes recognising up-regulated or down-regulated genes.

Abstract: The invention relates to process for in vitro prediction of a potentially allergenic substance wherein monocytes and/or macrophages and/or myelomonocytic cell lines are cultivated in the presence of the substance and interferon-?, whereby productions of cytokines and/or neopterin are increased and measured. The allergic reaction may be estimated by measuring up regulated or down-regulated genes chosen from G1P2, OASL, IFIT1, TRIM22, IFI44L, MXI, RSAD2, IFIT3, IFITM1, IFIT2, C 33.28 HERV-H protein mRNA, IFITM3, XK, GPR15, MT1G, MT1B; MT1A, ADFP, IL8, MT1E, MT1F, MT1H, SLC30A1, SERPINB2, CD83, TncRNA or expression products from them are measured. The invention also relates to a reagent kit for use in the process comprising interferon-? and reagents which recognise cytokines preferably each of IL-8 and neopterin and/or reagents such as probes recognising up-regulated or down-regulated genes.

Abstract: The present invention relates to a process for in vitro evaluation, of a potentially allergenic or tissue irritating sub-stance whereby test cells are cultivated in the presence of the substance, and the presence of up regulated genes chosen from G1P2, OASL, IFIT1, TRIM22, IFI44L, MXI, RSAD2, IFIT3, IFITM1, IFIT2, SPR, GNB2, XK, IFITM3, C 33.28 HERV-H protein mRNA, IFITM3, XK, GPR15, MT1G, MT1B; MT1A, ADFP, JL8, MT1E, MT1F, MT1H, SLC30A1, SERPINB2, CD83, TncRNA or expression products from them are measured. The invention also regards use of the expression products from one or more of the genes for in vitro analysis of allergy or tissue irritation. It also relates to a probe comprising at least three nucleic acids, preferably 3-40, especially 5-15 chosen from RNA complementary to the RNA corresponding to any of the genes and the use thereof for in vitro analysis of allergy or tissue irritation.

Abstract: The invention concerns a process for in vitro evaluation of a potentially allergenic or tissue irritating substances, characterized in that blood cells are cultivated in the presence of serial dilutions of the substance whereby the highest concentration of the substance being non toxic to the cells is serial diluted, cell proliferation is measured and the presence of cytokines is measured whereby: the presence of one or more of the alarm cytokines of class 0 only is an indication of tissue damage and chemical toxic effects; the presence of one or more alarm cytokines of class 0 and possibly one or more cytokines of class IV type but not neopterin is an indication of delayed type hypersensitivity such as cellular immunity, delayed allergy and contact eczema; and the presence of one or more alarm cytokines of class 0 and at least neopterin and possibly one or more cytokines of class I is an indication of immediate type hypersensitivity such as asthma, hay fever, urticaria and rhinitis.