Abstract: The present invention relates to a method for detecting nucleic acids by fluorescent PCR. The method combines primer-activated polymerization reaction and specific fluorescence-labeled probe, which detects the target nucleic acid with high selectivity and high specificity.
Abstract: The invention provides a method for detecting miRNA based on polymerase chain reaction comprising EvaGreen dye, and the use of EvaGreen dye in elevating the binding specificity of primers to templates in PCR.
Type:
Grant
Filed:
December 8, 2011
Date of Patent:
March 22, 2016
Assignee:
BIOVUE TECHNOLOGY LTD
Inventors:
Hongfei Wu, Honghoi Ting, Zuxun Gong, Ruoying Tan
Abstract: The invention provides a method for detecting miRNA based on polymerase chain reaction comprising EvaGreen dye, and the use of EvaGreen dye in elevating the binding specificity of primers to templates in PCR.
Type:
Application
Filed:
December 8, 2011
Publication date:
October 2, 2014
Applicant:
BIOVUE TECHNOLOGY LTD.
Inventors:
Hongfei Wu, Honghoi Ting, Zuxun Gong, Ruoying Tan