Abstract: A reagent containing a purified horseshoe crab Factor C, particularly a recombinantly produced Factor C, and a surfactant can be used in a sensitive, rapid, and reproducible assay to detect endotoxin.
Abstract: A method for identifying and quantifying chymopapain-specific IgE antibody levels in patient serum comprising binding chymopapain-specific IgE, if any, in the serum with chymopapain adhering to an insoluble support, conjugating the serum IgE with a labeled anti-IgE antibody, and measuring the level of labeled compound bound to the insoluble support or in the solution removed therefrom. Special reagents and their manufacture are also disclosed.
Type:
Grant
Filed:
May 25, 1990
Date of Patent:
January 28, 1992
Assignee:
BioWhittaker, Inc.
Inventors:
Emanuel Calenoff, Ruth M. Jones, Yuh-Geng Tsay, Myron A. Beigler