Abstract: A genetically modified Komagataella phaffii yeast cell for expression of a Protein or Polypeptide of Interest (POI). comprising in its genome a recombinant nucleic acid sequence encoding a POI, and a genetic modification in the open reading frame at any one or more of position 949930 on chromosome 1 (genbank LT962476.1), position 65654 on chromosome 2 (genbank LT962477.1), position 1303485 on chromosome 4 (genbank LT962479.1), position 1323758 on chromosome 1 (genbank LT962476.1) and/or position 1491140 on chromosome 4 (genbank LT962479.1).
Type:
Application
Filed:
December 29, 2021
Publication date:
December 5, 2024
Applicant:
BISY GMBH
Inventors:
Anton GLIEDER, Claudia RINNOFNER, Stefan ERTL, Katharina EBNER, Moritz BÜRGLER, Astrid RADKOHL
Abstract: The present invention relates to an optimized metabolic pathway design in P. pastoris. In particular, to a recombinant polycistronic expression construct for stable expression of multiple genes of interest in a yeast cell, preferably in P. pastoris.
Type:
Grant
Filed:
October 28, 2015
Date of Patent:
March 3, 2020
Assignee:
BISY GmbH
Inventors:
Martina Geier, Pia Fauland, Anton Glieder