Abstract: The invention provides a method of selectively amplifying a detection target nucleic acid by inhibiting amplification of a detection non-target nucleic acid (e.g., wild-type gene) in a test sample by using, in a nucleic acid amplification reaction, an oligonucleotide analog containing one or more kinds of one or more unit structures of various nucleoside analogs represented by the following formula (I): wherein the symbols are as defined in the DESCRIPTION, and the like, or a salt thereof, as a clamp nucleic acid, and detecting the amplified nucleic acid.
Type:
Grant
Filed:
September 27, 2013
Date of Patent:
April 9, 2019
Assignee:
BNA INC.
Inventors:
Takeshi Imanishi, Ayako Orita, Ikuya Ban
Abstract: The invention provides a method of selectively amplifying a detection target nucleic acid by inhibiting amplification of a detection non-target nucleic acid (e.g., wild-type gene) in a test sample by using, in a nucleic acid amplification reaction, an oligonucleotide analog containing one or more kinds of one or more unit structures of various nucleoside analogs represented by the following formula (I): wherein the symbols are as defined in the DESCRIPTION, and the like, or a salt thereof, as a clamp nucleic acid, and detecting the amplified nucleic acid.
Type:
Application
Filed:
September 27, 2013
Publication date:
August 27, 2015
Applicant:
BNA INC.
Inventors:
Takeshi Imanishi, Ayako Orita, Ikuya Ban