Abstract: A method of isolating purified plasmid DNA includes the steps of providing cells containing plasmid DNA and lysing the cells with an alkyldimethylphosphine oxide (APO) detergent to produce a mixture, centrifuging the cell and APO mixture, reserving a supernatant containing purified plasmid DNA and isolating the purified plasmid DNA, from the supernatant. Isolation and purification occurs in the absence of hazardous materials such as phenol and chloroform. Prior to mixing, the APO detergent is preferably combined with a strong base to produce a stable solution.
Type:
Grant
Filed:
August 26, 1994
Date of Patent:
April 29, 1997
Assignee:
Board of Regents For Northern Illinois Univ.
Inventors:
Gordon C. Kresheck, Mitchell Altschuler