Abstract: The present invention relates to novel purified and isolated nucleotide sequences encoding mammalian Ca2+/calmodulin stimulated phosphodiesterases (CaM-PDEs) and cyclic-GMP-stimulated phosphodiesterases (cGS-PDEs). Also provided are the corresponding recombinant expression products of said nucleotide sequences, immunological reagents specifically reactive therewith, and procedures for identifying compounds which modulate the enzymatic activity of such expression products.

Abstract: The present invention relates to novel purified and isolated nucleotide sequences encoding mammalian Ca2+/calmodulin stimulated phosphodiesterases (CaM-PDEs) and cyclic-GMP-stimulated phosphodiesterases (cGS-PDEs). Also provided are the corresponding recombinant expression products of said nucleotide sequences, immunological reagents specifically reactive therewith, and procedures for identifying compounds which modulate the enzymatic activity of such expression products.

Abstract: The Apa I restriction fragment of the human erythropoietin gene, for producing high titers of biologically active hormone from stably transfected cell lines.

Abstract: The present invention relates to novel purified and isolated nucleotide sequences encoding mammalian Ca2+/calmodulin stimulated phosphodiesterases (CaM-PDEs) and cyclic-GMP-stimulated phosphodiesterases (cGS-PDEs). Also provided are the corresponding recombinant expression products of said nucleotide sequences, immunological reagents specifically reactive therewith, and procedures for identifying compounds which modulate the enzymatic activity of such expression products.

Abstract: An MRI apparatus and method useful for both industrial applications and medical applications is provided. The apparatus and procedures are capable of estimating the value of a continuous property, such as concentration, viscosity or the like by interpolating or extrapolating from a model derived from training sets of data representing measurements of samples with known properties. A number of techniques are provided for objectifying the analysis. Cluster analysis techniques can be used to supplement, assist or replace subjective judgments by trained operators. Calculations or judgments regarding similarity can be made with respect to stored libraries of signatures, particularly where the library of stored signatures is obtained objectively, e.g., using cluster analysis, standardization and calibration. The signatures can be expanded signatures which include non-MR as well as MR data.

Abstract: The present invention relates to novel purified and isolated nucleotide sequences encoding mammalian Ca2+/calmodulin stimulated phosphodiesterases (CaM-PDEs) and cyclic-GMP-stimulated phosphodiesterases (cGS-PDEs). Also provided are the corresponding recombinant expression products of said nucleotide sequences, immunological reagents specifically reactive therewith, and procedures for identifying compounds which modulate the enzymatic activity of such expression products.

Abstract: A procedure is disclosed for automatically constructing wrappers for performing information-extraction from sites such as Internet resources that display relevant information, interspersed with extraneous text fragments, such as HTML formatting commands or advertisements. The procedure has three basic steps. First, a set of example pages are collected with a subroutine named GatherExamples. Gather Examples is provided with information describing how to pose example queries to the site whose wrapper is to be learned. Second, these example pages are labeled by a subroutine named LabelExamples—i.e., the information to be extracted from each example is identified for use in the third step. The LabelExamples subroutine uses a general framework for labeling pages using site-specific heuristics called recognizers, as well as allowing users to correct and modify the recognized instances. Finally, the labeled example pages are passed to a BuildWrapper subroutine, which constructs a wrapper.

Abstract: There is disclosed an apparatus for real-time imaging of cortical intrinsic signals or imaging tumor tissue or the margins, dimensions and grade of tumor tissue, comprising, a means for obtaining analog video images and a means for processing the analog video signals into either an averaged control frame or a subsequent frame, a means for acquiring and analyzing a plurality of subsequent frames and averaged control frame to provide a difference image, wherein the difference image is processed to account for movement and noise and amplify the changes across a dynamic range of the apparatus, and a means for displaying the difference image alone or superimposed over an analog video image.

Abstract: Method of diagnosing arterial chlamydial granuloma by detecting in a biological sample both a first marker associated with Chlamydia pneumoniae and a second marker associated with arterial granuloma. Therapeutic composition for treating arterial chlamydial granulomatous disease, including an anti-Chlamydia pneumoniae agent and a granuloma inhibitor.

Abstract: The present invention provides novel purified and isolated nucleotide sequences encoding the cGMP-binding, cGMP-specific phosphodiesterase designated cGB-PDE. Also provided by the invention are methods and materials for the recombinant production of cGB-PDE polypeptide products and methods for identifying compounds which modulate the enzymatic activity of cGB-PDE polypeptides.

Abstract: Human pancreatic islet cell glutamic acid decarboxylase (GAD), an autoantigen involved in the development of insulin-dependent diabetes mellitus (IDDM), has been cloned, sequenced and expressed by recombinant means. Recombinant human islet cell GAD polypeptides and antibodies specific to the GAD polypeptides can be used in methods of diagnosis and treatment, including use in immunoadsorptive therapy and the induction of immune tolerance.

Abstract: The present invention provides novel recombinant nucleic acid vectors which may be used to produce .alpha.-globin as well as other proteins of interest in quantity in the red blood cells of transgenic animals or cell cultures of erythroid lineage. The present invention also provides for the transgenic animals which contain these recombinant nucleic acid vectors. The vectors of the invention comprise at least one of the major DNase I hypersensitivity sites associated with the .beta.-globin locus together with a gene of interest. According to various embodiments of the invention, the vectors may be used to create transgenic animals or to transfect cells in culture. In a specific embodiment of the invention, a vector which comprises two DNase I hypersensitivity sites together with the human .alpha.-globin gene is used to create transgenic animals which produce human .alpha.-globin protein in erythroid tissues, including red blood cells.

Abstract: The present invention relates to novel purified and isolated nucleotide sequences encoding mammalian Ca.sup.2+ /calmodulin stimulated phosphodiesterases (CaM-PDEs) and cyclic-GMP-stimulated phosphodiesterases (cGS-PDEs). Also provided are the corresponding recombinant expression products of said nucleotide sequences, immunological reagents specifically reactive therewith, and procedures for identifying compounds which modulate the enzymatic activity of such expression products.

Abstract: A virtual retinal display utilizes photon generation and manipulation to create a panoramic, high resolution, color virtual image that is projected directly onto the retina of the eye without creating a real or an aerial image that is viewed via a mirror or optics. The virtual retinal display includes a source of photons, the photons being modulated with video information and scanned in a raster type of pattern directly onto the retina of the user's eye. The photon generator may utilize coherent or non-coherent light. Further, the photon generator may utilize color light generators so as to scan a colored virtual image directly onto the retina of the user's eye. The virtual retinal display may also include a depth accommodation cue to vary the focus of scanned photons rapidly so as to control the depth perceived by a user for each individual picture element of the virtual image.

Abstract: This invention provides methods to locate and plan the retrieval of data from networked information sources in response to a user query. The methods utilize descriptions of the information sources, the information domain of the sources, and of the query. The methods of this invention integrate both legacy systems and full relational databases with an efficient, domain-independent, query-planning algorithm, reason about the capabilities of different information sources, handle partial goal satisfaction i.e., gather as much data as possible when all that the user requested cannot be gathered, are both sound and complete, and are efficient.

Abstract: There is disclosed a diagnostic sensor device comprising a plurality of sensor probes, a detection device, and a computer for analyzing the signals generated from each sensor probe by multivariate statistical analysis. The plurality of sensor probes comprise a substrate that allows transmission of a signal and a partially selective surface coating the substrate wherein the partially selective surface binds proteins from a biological fluid by multiple, noncovalent interactions, and with the proviso that the partially selective surfaces of each sensor probe in the diagnostic sensor device be different. The diagnostic sensor device can be used as a method for diagnosing disease states in humans and animals, wherein the disease states are characterized by altered protein character and behavior of a biological fluid.

Abstract: In vivo assays for selecting candidate therapeutics for inhibiting amyloidoses, such as congophilic and fibrillar .beta./A4 amyloid deposition in brain. A candidate reagent is administered to a first rat in a first infusate comprising .beta./A4 peptide and perlecan by continuous infusion into the hippocampus for at least one week. The candidate reagent is selected as a candidate therapeutic for inhibiting congophilic and fibrillar .beta./A4 amyloid deposition in brain if the first infusate diminishes congo red and thioflavin S staining indicative of amyloid deposition adjacent to the infusion site, as compared with a second rat receiving a second infusate consisting essentially of .beta./A4 peptide and perlecan.

Abstract: Genomic and cDNA sequences coding for a protein having substantially the same biological activity as human protein C are disclosed. Recombinant plasmids and bacteriophage transfer vectors incorporating these sequences are also disclosed.

Abstract: Genomic and cDNA sequences coding for a protein having substantially the same biological activity as human protein C are disclosed. Recombinant plasmids and bacteriophage transfer vectors incorporating these sequences are also disclosed.

Abstract: The invention relates to in vivo peripheralization of CD34+ cells by administering anti-VLA-4 antibodies or anti-VCAM-1 antibodies.