Abstract: Certain embodiments provide a method for crystallizing a GPCR. The method may employ a fusion protein comprising: a) a first portion of a G-protein coupled receptor (GPCR), where the first portion comprises the TM1, TM2, TM3, TM4 and TM5 regions of the GPCR; b) a stable, folded protein insertion; and c) a second portion of the GPCR, where the second portion comprises the TM6 and TM7 regions of the GPCR.

Abstract: Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.

Abstract: The present invention provides methods for detecting changes in tryptophan concentrations in a cell and methods for identifying agents that modulate cellular tryptophan concentrations. In particular, the present invention provides methods for detecting cellular exchange between tryptophan and kynurenine, and methods for identifying agents that modulate this exchange. The present invention also provides methods for treating a disease associated with immunosuppression in a subject in need thereof. In particular, the present invention is directed toward a method of treating a disease associated with immunosuppression comprising contacting the disease with an agent that modulates cellular Trp/kynurenine exchange. Furthermore, the present invention provides methods for identifying an agent that modulates an immunosuppression.

Abstract: Detection of toxic gluten oligopeptides refractory to digestion and antibodies and T cells responsive thereto can be used to diagnose Celiac Sprue.

Abstract: A long-range lightning detection and characterization system and method. Electromagnetic radiation produced by a lightning strike is sensed at a sensing location and a measured signal representative of the strike is produced that defines an amplitude versus time. A set of reference data containing waveforms comparable to the measured signal is established. The set of reference data defines a set of reference amplitudes versus time, representative of one or more predetermined reference lightning strikes at various predetermined distances, where for each of the distances, the reference lightning strikes have associated therewith various predetermined day/night percentages. The measured signal is compared with the reference data, the comparison including finding a correlation between the measured signal and a correlating waveform in the reference data.

Abstract: Disclosed are peptides which inhibit the enzymatic activity of tyrosinase as well as formulations and methods for their use in the reduction of skin pigmentation, and methods of administering the inhibitory peptides in a topical formulation. Methods of skin treatment are also provided, the methods further including use of a peptide characterized by the amino acid sequence SFLLRN (SEQ ID NO: 1).

Abstract: This invention concerns a process to convert a hydroxyl group (bold in R3C—OH) in a tigliane-type compound to a hydrogen (bold in R3C—H) to obtain deoxytigliane-type compounds or structural or functional analogs thereof. The process has wide application particularly to produce specific biologically active compounds in quantity for use as pharmaceuticals. In particular the process can be used to convert phorbol to a 12-deoxytigliane (prostrating which is a therapeutic lead for the treatment of AIDS. New compositions of matter are also disclosed.

Abstract: Recombinant adeno-associated viral (AAV) capsid proteins are provided. Methods for generating the recombinant adeno-associated viral capsid proteins and a library from which the capsids are selected are also provided.

Abstract: An optical waveguide gyroscope includes at least one optical coupler configured to receive a first optical signal at a first port, to transmit a second optical signal to a second port, and to transmit a third optical signal to a third port. The optical waveguide gyroscope further includes a plurality of resonant waveguides optically coupled to the second port and the third port. The resonant waveguides are generally adjacent to one another and optically coupled to one another. At least a portion of the second optical signal propagates from the second port to the third port by propagating through the plurality of resonant waveguides, and at least a portion of the third optical signal propagates from the third port to the second port by propagating through the plurality of resonant waveguides.

Abstract: Biological thick tissue such as skeletal and cardiac muscle is imaged by inserting a probe into the tissue and scanning the tissue at a sufficiently fast rate to mitigate motion artifacts due to physiological motion. According to one example embodiment, such a probe is part of a system that is capable of reverse-direction high-resolution imaging without staining or otherwise introducing a foreign element used to generate or otherwise increase the sensed light. The probe includes a light generator for generating light pulses that are directed towards structures located within the thick tissue. The light pulses interact with intrinsic characteristics of the structures to generate a signal such as SHG or intrinsic fluorescence. Reliance on intrinsic characteristics of the structures is particularly useful for applications in which the introduction of foreign substances to the thick tissue is undesirable.

Abstract: An optical sensor includes an optical coupler configured to receive a first optical signal and to split the first optical signal into a second optical signal and a third optical signal. The optical sensor further includes a Bragg fiber in optical communication with the optical coupler. The second optical signal and the third optical signal counterpropagate through the Bragg fiber and return to the third port and the second port, respectively.

Abstract: A biologically inspired climbing device with toes and spines is provided. The device has toes capable of moving independently from each other. Each toe distinguishes a compliant linkage, which has non-compliant parts and compliant parts. Spines are distributed over each of the toes. The spines have a diameter that is less than or equal to a diameter of an asperity of a surface. The toes with spines could be arranged into one or more feet. The feet could then be organized in legs enabling the device to climb a surface in a gait pattern. The device uses low power and is quiet, leaves no traces or tracks behind, works well on smooth, rough, uneven, porous and dirty surfaces, and is able to carry and support its own body weight.

Abstract: Method and apparatus which uses harmonic cantilevers, such as used in atomic force microscopy, to detect variations in the attractive and repulsive forces on a solid surface as a result of macromolecular binding, for example, hybridization of a single stranded DNA molecule attached to the surface with another DNA molecule. The complexed macromolecule is less flexible than an uncomplexed molecule. It will typically have more negative charge due to amino acids or DNA monomers. Both stiffness of the surface and the attractive capillary forces will change after binding and may be detected. The present methods and materials enable ultraflat surfaces for the macromolecule deposition, and may include the use of a gold-coated mica substrate and a self-assembling monolayer.

Abstract: A radiation imaging device (10). The radiation image device (10) comprises a subject radiation station (12) producing photon emissions (14), and at least one semiconductor crystal detector (16) arranged in an edge-on orientation with respect to the emitted photons (14) to directly receive the emitted photons (14) and produce a signal. The semiconductor crystal detector (16) comprises at least one anode and at least one cathode that produces the signal in response to the emitted photons (14).

Abstract: Compositions and methods are provided that relate to the bioremediation of chlorinated ethenes, particularly the bioremediation of vinyl chloride by Dehalococcoides-like organisms. An isolated strain of bacteria, Dehalococcoides sp. strain VS, that metabolizes vinyl chloride is provided; the genetic sequence of the enzyme responsible for vinyl chloride dehalogenation; methods of assessing the capability of endogenous organisms at an environmental site to metabolize vinyl chloride; and a method of using the strains of the invention for bioremediation.

Abstract: A method for validating a nanotube logic network. The nanotube logic network is separated into regions based on a conductivity of the respective region. Potential paths through adjoining regions of the nanotube logic network are determined. Boolean path functions for each potential path are determined. If the Boolean path functions of the potential paths are equivalent to the intended logic function, then the nanotube logic network is immune to misaligned carbon nanotubes.

Abstract: According to example embodiments for use in a communication system that is adapted to permit the users to transmit data simultaneously via shared frequency and spatial resources, an allocation of user transmission rates involves setting and maintaining the transmission rates of the users to at least a minimum user transmission rate to provide an expected minimum quality of communication for each of the users. These rates of the users are incrementally adjusted by iteratively changing the transmission rate of each user as a function of a resulting vector of transmit powers ensuing from the increased transmission rate, a degree of transmission-rate-allocation unfairness relative to the transmission rates of all the users, and a power-based selection criteria. With such an approach, the rates are fairly allocated without a disproportionate allocation of system bandwidth. Various embodiments are applicable to a variety of communication applications including OFDM or CDMA communication systems.

Abstract: Data is stored in a quantum-well type structure with double gate control. According to an example embodiment, a transistor-based data storage circuit includes a gate, a back gate and a semiconductor channel between the gate and the back gate. Carriers are stored in a storage pocket structure in the channel, in response to biases applied to the gate and back gate. Current passing through the channel is sensed and used to detect the stored carriers and, correspondingly, a memory state of the storage circuit.

Abstract: Devices, bandages, kits and methods are described that can control or regulate the mechanical environment of a wound to ameliorate scar and/or keloid formation. The mechanical environment of a wound includes stress, strain, and any combination of stress and strain. The control of a wound's mechanical environment can be active, passive, dynamic, or static. The devices are configured to be removably secured to a skin surface in proximity to the wound site and shield the wound from endogenous and/or exogenous stress.

Abstract: Embodiments of the present disclosure include hybrid quantum dot/protein nanostructure, hybrid quantum dot/protein nanostructure systems, methods of using hybrid quantum dot/protein nanostructures, and the like.