Abstract: A scanning probe microscope is used to pattern a layer of resist, and the pattern is transferred to a substrate. First, an underlayer formed of, for example, polyimide and a top layer formed of, for example, amorphous silicon are deposited on the substrate. A pattern is formed on the top layer using the tip of the cantilever in a scanning probe microscope. The pattern may consist of an oxide formed by an electric field at the cantilever tip. The top layer is then etched using the pattern as a mask and using an etchant that is selective against the underlayer. The underlayer is then etched using an etchant that is selective against the top layer and substrate. The substrate is etched with an etchant that removes the top layer but is selective against the underlayer. Finally, the underlayer is removed.

Abstract: An optical system is disclosed which focuses a polychromatic source to an extended focal pencil. The implementation makes use of two holographic optical elements (HOEs) fabricated and aligned to form a deliberate longitudinal color dispersion, but to alleviate lateral chromatic effects. Consequently, the HOE doublet focuses different wavelengths of the source to different locations along the optical axis. The strong intensity of the focused wavelengths dominate at each location, so that the overall beam has a near-diffraction-limited 1/e.sup.2 spot size and suffers only relatively weak background illumination. An alternative optical system using bulk lenses and several possible applications for the device are also described.

Abstract: Magnetic recording media comprising an ordered, ultra-high density array of 500 .ANG. diameter circular magnetic thin film islands on a substrate. The magnetic material supports magnetization perpendicular to the film plan, and each circular island comprises a single magnetic domain and a single information storage bit. An areal bit density of 10.sup.11 bits/in.sup.2 can be achieved by such an array. The magnetic array is generated using a single level mask comprised of a self-ordering polymer array, either an array of polymer spheres or a regular array of polymeric blocks in a phase-separating polymer film.

Abstract: The present invention provides a constrained stochastic dynamical method for simulating the motion of a molecular system. The method simulates the motions of atoms within the molecular system by evaluating first order force expressions for all the atoms over a series of time steps. The force expressions include terms for frictional forces, non-covalently interatomic forces, thermal noise forces, and covalent constraining forces. Relatively long time steps can be used because the fast motions (such as vibrations associated with covalent bonds) are not explicitly considered. Rather, the fast molecular motions associated with covalent bonds are averaged around their equilibrium bond lengths and, in some cases, bond angles. This is implemented by introducing (1) thermal noise forces, and (2) constraining forces fixing the relative positions of some atoms based upon their covalent bonds.

Abstract: A photolithography system includes a plurality of cantilevers, preferably formed in a silicon wafer. Each cantilever includes a tip located near the free end of the cantilever and a waveguide which extends along the length of the cantilever and intersects the tip. An aperture is formed at the apex of the tip so that light travelling through the waveguide may exit the tip. A light switch is included in the waveguide to control the passage of light to the tip of the cantilever.The array of cantilevers is positioned adjacent a wafer which is to be lithographed, in the manner of an atomic force microscope operating in the attractive mode. Each cantilever is a compound structure, including a thick portion and a thin portion, the latter having a preselected mechanical resonant frequency.

Abstract: By providing a focal region of light onto a particle, a laser-based light source can provide enough radiation pressure to position the particle at any desired location in space. In one application, the particle can be a micrometer-sized bead, called a handle, attached to a sample. When the sample under examination, such as an actin filament, interacts with other molecules, such as myosin, the forces generated may displace the sample, and thus the handle, out of its original position. To correct for the off-target position (or in other words, to increase the stiffness of the handle), a feedback loop that utilizes a quadrant photodiode detector and a focal region location means such as an acousto-optic modulator or galvanometer mirror is incorporated in the optical trap system. Use of two other light sources for brightfield illumination and epifluorescence allows the simultaneous viewing of the sample in real time. In other embodiments, the optical trap system can trap and manipulate particles.

Abstract: Recombinant mycobacterial vaccine vehicles capable of expressing DNA of interest which encodes at least one protein antigen for at least one pathogen against which an immune response is desired and which can be incorporated into the mycobacteria or stably integrated into the mycobacterial genome. The vaccine vehicles are useful for administration to mammalian hosts for purposes of immunization. A recombinant vector which replicates in E. coli but not in mycobacteria is also disclosed. The recombinant vector includes 1) a mycobacterial gene or portions thereof, necessary for recombination with homologous sequences in the genome of mycobacteria transformed with the recombinant plasmid; 2) all or a portion of a gene which encodes a polypeptide or protein whose expression is desired in mycobacteria transformed with the recombinant plasmid; 3) DNA sequences necessary for replication and selection in E. coli; and 4) DNA sequences necessary for selection in mycobacteria (e.g., drug resistance).

Abstract: This invention relates to an improvement in promoting the rate of association for high specificity binding pairs used in a variety of industrial, research and medical applications. These pairs include enzyme/substrate, complementary polynucleotide and antibody/antigen combinations. In one specific embodiment, this invention relates to the acceleration of nucleic acid hybridization by heterogeneous nuclear ribonucleoproteins [hnRNPs]. In another specific embodiment, this invention relates to the acceleration of nucleic acid hybridization by a cationic detergent.

Abstract: The penetration depth of an intraluminal device, such as a transesophageal probe or a vascular catheter, is monitored by totalizing incremental advancement and withdrawal of the device over time. For a first embodiment, the devices are provided with axially spaced-apart markings along at least a portion of their lengths. The penetration depth of such intraluminal devices into a body lumen is monitored by detecting passage of the markings past a fixed location relative to the body lumen. Usually, the markings will be optically detected in a manner which permits forward and retrograde motion to be distinguished. Thus, the net totalization of the markers will correspond to the actual penetration distance in real time. In an alternate embodiment, the incremental advancement and withdrawal may be followed by use of a contact roller. In either case, the distance can be displayed by itself or in combination with a real time image, with the distance and image optionally being recorded.

Abstract: A microelectronic substrate patterning system and method uses two or more copies of a mask pattern in the radiation path between the radiation source and the microelectronic substrate. The two or more copies are axially displaced from one another along the radiation path and are in axial alignment with each other along the radiation path. Thus, the mask pattern is imaged onto the microelectronic substrate as a result of interaction of the radiation with the multiple copies of the mask pattern in the radiation path. The mask pattern thereby images at an increased depth to focus compared to a single copy of the mask pattern. The two or more copies of the mask pattern may be used to increase the depth of focus of transmissive and reflective microelectronic substrate imaging systems. The mask copies may be identical or may be biased relative to the desired feature dimensions.

Abstract: Adaptive decision feedback equalizer apparatus for processing information stored on disk or tape media or the like including a data input buffer (34), a gain acquisition circuit (42), a timing acquisition circuit (40) operative to generate timing error signals for controlling the sampling phase of the read signals input to the input buffer, a synchronizing circuit (44) for generating sync detect signals and polarity signals, an FIR filter (36) for generating linear filter output signals, register means (39), feedforward update logic (38) for adjusting the equalizer coefficient signals to develop updated coefficient signals, a dual ported RAM (50) for storing a plurality of the equalizer coefficient signals, feedback logic (48) responsive to the linear filter output signals, equalizer coefficient signals obtained from the RAM, and train data signals, and operative to compute the equalizer error signals and equalizer output signals, feedback update logic (52) for adjusting the values of the coefficient signals

Abstract: Methods and compositions are provided for producing heterologous proteins in a micrcorganism host, particularly bacterium, without fusion to an endogenous protein. Particularly, the heterologous gene may be inserted into a vector comprising an endogenous gene, where the heterologous gene is preceded by a ribosomal binding site. A heterologous enzyme functional in a bacterial host is demonstrated.

Abstract: The holographic element of the present invention includes a holographic grating pattern that is impressed in the media of the element utilizing beams of electromagnetic energy of wavelength .lambda..sub.o. The holographic grating pattern is capable of manipulating a beam of electromagnetic energy having a wavelength .lambda..sub.c at which the media is not necessarily suitable for recording. The method for manufacturing the holographic element utilizes an object beam and a reference beam having aspherical wavefronts of wavelength .lambda..sub.o to create the grating pattern in the media. In the preferred embodiment, the aspherical object beam and reference beam are created utilizing parent holographic devices recorded with spherical beams. An alternative embodiment of the present invention is a doublet holographic device which incorporates two holographic elements that are formed in accordance with the present invention to manipulate a beam of electromagnetic energy of wavelength .lambda..sub.

Abstract: An elegant design for an optical modulator having a high Q is described. The modulator is made up of a straight-forward microwave guide within which a block of a monocrystalline electro-optic material such as lithium niobate is positioned. The modulator is dimensioned to support sequentially standing waves of modulating energy within the desired bandwidth. Optical radiation to be modulated is introduced into the crystal. In one embodiment designed particularly for the 2-15 GHz range, the amount of crystalline material necessary to interact with the incoming radiation is selected to be a minimum, and in another embodiment designed for the 15-75 GHz frequency range, a zig-zag reflection pattern is provided within the crystal.

Abstract: Methods and compositions are provided for producing and utilizing nucleic acid and peptide sequences associated with cofactors which bind to transcription factors to enhance transcriptional activity of the transcriptional factors and maintain the transcriptional factors as dimers. The compositions can be used for modulating expression of genes, particularly coordinately regulated genes, as evidenced by the combination of the transcription factors HNF-1.alpha. and -1.beta. with the cofactor DCoH.

Abstract: A monolithic crystalline material for quasi-phase-matching is described. The material includes a plurality of wafers of an odd multiple of coherence length thickness, having their faces bonded together by diffusion bonding. The wafers are oriented relative to one another to alternate their signs of nonlinear susceptibility. The invention also includes a method for producing optical radiation of a selected frequency by quasi-phase-matching, several specific methods and materials based on the discovery responsible for the invention, a generator for radiation of 60 THZ frequency of significant power, and a method of fabricating the material.

Abstract: A modulator adapted to be acoustically coupled to an optical fiber for applying acoustic waves across the fiber at a spatial and phase angle with respect to one another to compress and decompress the optical fiber at said spatial angle responsive to an electrical signal whereby to modulate the polarization state of an optical wave travelling past said modulator. An optical communication system in which light waves are transmitted from a transmitting end to a receiving end and whose polarization is modulated by an acoustic modulator responsive to an input signal and the output signal is demodulated to recover the signal.

Abstract: A method of assessing a cell's susceptibility to cell-damaging energy, such as ionizing radiation and heat, is disclosed. The method is based on measurable changes in voltage-dependent potassium channel currents in the cell in response to the energy. Also disclosed is a method for screening drugs which are effective to sensitize a cell to cell-damaging radiation.

Abstract: A microminiature cantilever structure is provided having a cantilever arm with a piezoresistive resistor embedded in at least the fixed end of the cantilever arm. Deflection of the free end of the cantilever arm produces stress in the base of the cantilever. That stress changes the piezoresistive resistor's resistance at the base of the cantilever in proportion to the cantilever arm's deflection. Resistance measuring apparatus is coupled to the piezoresistive resistor to measure its resistance and to generate a signal corresponding to the cantilever arm's deflection. The microminiature cantilever is formed on a semiconductor substrate. A portion of the free end of the cantilever arm is doped to form an electrically separate U-shaped piezoresistive resistor. The U-shaped resistor has two legs oriented parallel to an axis of the semiconductor substrate having a non-zero piezoresistive coefficient.

Abstract: Novel methods and microorganisms are provided, where novel genetic mammalian cell invasive capability is imparted to a microorganism by the introduction of an exogenous inv gene. The resulting organisms are then capable of binding to mammalian cells and are transferred to the cytoplasm. Other novel genetic capabilities may be imparted to the unicellular microorganism, which may serve as a vaccine for one or more pathogens or may introduce genetic capabilities or foreign molecules into a mammalian host cell. The sequences may be used for an in vitro screen for pathogenicity.