Abstract: A process for the preparation of an isoflavone from a 2'-hydroxydeoxybenzoin(I) is described. The process uses prepared N,N'-dimethyl(chloromethylene) ammonium chloride as a reactant with (I) in borontrifluoride etherate or other Lewis acid as a solvent. The isoflavones are known biologically active compounds.

Abstract: DNA encoding glycoproteins gD, gI and gE from Marek's disease herpesvirus is described The DNA is useful for probes to detect the DNA in the herpesvirus, for expression to produce the glycoproteins that can be used for producing the antibodies which specifically recognize the three glycoprotein antigens, and in the case of the latter two genes for potential insertion sites for foreign genes and as possible sites for gene inactivation to attenuate MDV field isolates for vaccine purposes.

Abstract: The cDNA encoding safener binding protein (SafBP), also referred to as SBP1, is set forth in FIG. 5 and SEQ ID No. 1. The deduced amino acid sequence is provided in FIG. 5 and SEQ ID No. 2. Methods of making and using SBP1 and SafBP to alter a plant's sensitivity to certain herbicides or a plant's responsiveness to certain safeners are also provided, as well as expression vectors, transgenic plants or other organisms transfected with said vectors and seeds from said plants.

Abstract: A bulk extrusion polymerization process to derive an aliphatic polyester from a suitable ester monomer, preferably .epsilon.-caprolactone. Novel reaction formulations containing less than 100 ppm water and having an acid value less than 0.5 mg KOH/g and preferably less than 0.2 mg KOH/g enable the polymerization of bulk monomer in an extruder in a resident time of less than 2 minutes. Different catalyst and initiator systems, processing protocols, and screw configurations lead to the preferred manufacture of poly(.epsilon.-caprolactone) polymer of number average molecular weight, M.sub.n, greater than 100,000. A novel poly(.epsilon.-caprolactone) intermediate optionally grafted to starch, is described which has significantly different processing and use characteristics as compared to a straight-chain polycaprolactone.

Abstract: An electronically controlled micro-dispensing apparatus (10 or 210) is described. The apparatus includes a liquid storage container (12), a propellant container (16), a control circuit (34 or 42) and an ejector (28). In a second embodiment, the liquid and propellant are in the same container (212). The control circuits control the length of pulse and the time duration between each pulse. By controlling these factors, the apparatus dispenses a precise amount of liquid over a set time period. The ejector allows for controlled dispensing and allows the liquid to be sprayed a greater distance. In the preferred embodiment, the apparatus are used to dispense a pheromone in an outdoor setting, such as an orchard, to control insects.

Abstract: A lockable and removable barrier assembly (10) includes a post (32) which is removably mounted in a sleeve (12). The bottom end (32B) of the post is mounted in the top end (12A) of the sleeve such that a securing opening (14) of the sleeve is aligned with a securing opening (35) of the post. A securing mechanism (42) is located in the inner chamber (32C) of the post. The securing mechanism includes a securing lever (52) which is pivotably mounted at the bottom end (52B) to a mounting bracket (44). The lever includes a securing lug (58) which extends through a securing opening in the post and sleeve to secure the post in position in the sleeve. The top end (52A) of the lever is held in the secured position by a lock (60) which extends through a hole in the end of the lever and through a hole in the locking bracket (48) mounted on the sidewall of the post in the inner chamber.

Abstract: An Arabidopsis acetyl-CoA carboxylase (ACCase) gene has been isolated and sequenced. The 10 kb gene encodes a 251 kD cytosolic ACCase isozyme. The nucleic acid sequence of the gene is set forth in SEQ ID No. 1 and has been accorded GenBank Accession No. L27074. The cDNA sequence and deduced amino acid sequence of the cDNA are set forth in SEQ ID Nos. 2 and 3, respectively. By controlling expression of the gene of the present invention, carboxylation of acetyl-CoA to produce malonyl-CoA may be controlled. Thus, by introducing constructs of the gene of the present invention in sense or anti-sense orientation, carboxylation of acetyl-CoA to produce malonyl-CoA may be increased or decreased. Consequently, fatty acid synthesis and elongation in plants and seeds which depends on malonyl-CoA may also be controlled.

Abstract: A method and apparatus (10) for separating materials having different size densities is described. The apparatus includes a trough (12), a dispersing and conveying screw means (40) and a fluid distribution manifold (26). The trough has upper and lower portions (12F and 12G) with the lower portion forming a pool area. The dispersing and conveying screw means has a conveying portion (46) and a dispersing portion (50). The conveying portion has flights (48) which move the first material (152) up the trough. The dispersing portion has paddles (52) which disperse the mixture (150) and convey the first material to the conveying portion. The fluid distribution manifold supplies both water and air to the inside (12I) of the trough through fluid orifices (28). In operation, the mixture is fed into the trough directly over the fluid orifices and the dispersing portion of the dispersing and conveying screw means. As the mixture enters the trough, the mixture is dispersed and forms an aqueous suspension with the water.

Abstract: A method and vaccine for treatment of pythiosis in humans and animals is described. In particular a vaccine comprising a mixture of extracellular and intracellular proteins is described. The vaccine enables cures of chronic pythiosis in some patients.

Abstract: Compositions incorporating a postemergence herbicide and a monosaccharide, particularly fructose, as a potentiator of the herbicide against weeds without decreasing tolerance of a crop plant to the herbicide are described. The compositions are used as a spray in water in a method for killing weeds.

Abstract: The present invention provides a process for the treatment of arthritis including the step of administering to an animal or human patient in need of such treatment a plurality of peptides each of which peptides contains a sequence of amino acid residues that is identical to or homologous to residues 110-121 or 152-161 of myelin basic protein. Novel peptides for use in that process are also provided.

Abstract: An Arabidopsis acetyl-CoA carboxylase (ACCase) gene has been isolated and sequenced. The 10-kb gene encodes a 251-kd cytosolic ACCase isozyme. The nucleic acid sequence of the gene is set forth in SEQ ID No. 1 and has been accorded GenBank Accession No. L27074. The cDNA sequence and deduced amino acid sequence of the cDNA are set forth in SEQ ID Nos. 2 and 3, respectively. By controlling expression of the gene of the present invention, carboxylation of acetyl-CoA to produce malonyl-CoA may be controlled. Thus, by introducing constructs of the gene of the present invention in sense or anti-sense orientation, carboxylation of acetyl-CoA to produce malonyl-CoA may be increased or decreased. Consequently, fatty acid synthesis and elongation in plants and seeds which depends on malonyl-CoA may also be controlled.

Abstract: A method and test kit for determining milk production in bovines is described. The acetyl coenzyme A synthetase (ACS) production is determined and related to milk production. The method is useful in selection and/or breeding to enhance milk production. Recombinant microganisms, plasmids and ACS is also described.

Abstract: A protein derivable from Actinobacillus pleuropneumoniae (APP) having a molecular size ranging between 43 Kd on SDS PAGE is described. The protein is soluble on sodium lauryl sarcosinate (SLS) and not heat modifiable. The protein can also be derived by recombinant methods. The protein in a vaccine provides immunity against multiple serotypes of APP.

Abstract: A bulk extrusion polymerization process to derive an aliphatic polyester from a suitable ester monomer, preferably .epsilon.-caprolactone. Novel reaction formulations containing less than 100 ppm water and having an acid value less than 0.5 mg KOH/g and preferably less than 0.2 mg KOH/g enable the polymerization of bulk monomer in an extruder in a resident time of less than 2 minutes. Different catalyst and initiator systems, processing protocols, and screw configurations lead to the preferred manufacture of poly(.epsilon.-caprolactone) polymer of number average molecular weight, M.sub.n, greater than 100,000. A novel poly(.epsilon.-caprolactone) intermediate optionally grafted to starch, is described which has significantly different processing and use characteristics as compared to a straight-chain polycaprolactone.

Abstract: A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe.

Abstract: A process for depositing an adherent polycrystalline diamond thin film on a glass substrate, by chemical vapor deposition (CVD) at 1 to 15 torr and low temperatures of the substrate of between about 350 to 600.degree. C. using hydrogen and methane and optionally carbon dioxide. The substrate has diamond particles deposited on it or is polished with diamond particles prior to CVD. The process produces films which are clear and adherent.

Abstract: A physical mixture which consists essentially of compositions of lysine monohydrochloride and potassium chloride alone or admixed with small amounts of succinic acid, in particular weight ratios, and which has a salty taste comparable to table salt (sodium chloride). The mixture masks the bitter aftertaste of the potassium chloride and can provide dietary lysine which is an essential amino acid.

Abstract: An analytical method for detecting the presence poly-beta-hydroxybutyrate (PHB), which can be used as an indicator of atherosclerotic risk is disclosed. In the method, antibodies to PHB are used to detect the PHB.

Abstract: A method for producing continuous and discontinuous fiber metal matrix composites (CFMMC). The method uses aerosolization of finely divided metal powders in a controlled atmosphere which prevents explosions to coat the fibers and then the metal coated fibers are consolidated to form the CFMMC. The composites are useful as heat sinks for electrical components and in applications where a structural reinforced metal matrix composite is needed.