Patents Assigned to Boehringer Ingelheim International FmbH
  • Method for introducing nucleic acids into higher eukaryotic cells
    Patent number: 5965404
    Abstract: Process and medium for the transfection of higher eukaryotic cells with DNA/polycation complexes, wherein a medium is used which contains ethyleneglycol and/or glycerol. The medium may also contain a substance which prevents the acidification of the endosomes, and/or a lower alcohol such as ethanol. The process is particularly suitable for the transfection of primary cells such as fibroblasts. Stably transformed cells can be obtained thereby, e.g. tumour cells for use as tumour vaccines.
    Type: Grant
    Filed: March 6, 1997
    Date of Patent: October 12, 1999
    Assignee: Boehringer Ingelheim International FmbH
    Inventors: Michael Buschle, Ernst Wagner, Wolfgang Zauner