Abstract: Provided herein are mycelium materials and methods for production thereof. In some embodiments, a mycelium material includes: a cultivated mycelium material including one or more masses of branching hyphae, wherein the one or more masses of branching hyphae may be disrupted or pressed and/or a bonding agent may be combined with the cultivated mycelium material. Methods of producing a mycelium material are also provided.

Abstract: This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.

Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the ?-mating factor (?MF) of Saccharomyces cerevisiae and a non-?MF signal peptide.

Abstract: Disclosed herein are modified strains for improving secretion of recombinantly expressed products secreted from a host organism with improved growth and productivity characteristics, as well as methods of using the modified strains.

Abstract: A method for manufacturing a garment includes gathering customer data from a plurality of customers, the customer data being associated with respective ones of a plurality of garment orders and including customer information regarding at least one customizable garment parameter and generating a schedule for producing a plurality of customized garments including analyzing the at least one customizable garment parameter for the at least some of the plurality of garment orders. The method further includes manufacturing the plurality of customized garments according to the schedule.

Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.

Abstract: The present disclosure provides improved long uniform recombinant protein fibers with desirable physical traits. The present disclosure also provides compositions derived from the long uniform recombinant protein fibers.

Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the ?-mating factor (?MF) of Saccharomyces cerevisiae and a non-?MF signal peptide.

Abstract: Disclosed herein are modified strains for reducing degradation of recombinantly expressed products secreted from a host organism and methods of using the modified strains. In some embodiments, to attenuate a protease activity in Pichia pastoris, the genes encoding enzymes the degrade proteases are inactivated or mutated to reduce or eliminate activity. In preferred strains, the protease activity of proteases encoded by PAS_chr4_0584 (YPS1-1) and PAS_chr3_1157 (YPS1-2) (e.g., polypeptides comprising SEQ ID NO: 66 and 67) is attenuated.

Abstract: Provided herein are scalable methods of processing wet-spun fiber comprising recombinant spider silk polypeptides to generate a three-dimensional crystalline lattice of beta-sheet structures in the fiber.

Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.

Abstract: An article of footwear includes an upper and a midsole coupled with the upper. The midsole includes at least a portion of a solid resilin material comprising a cross-linked recombinant resilin and a polar nonaqueous solvent.

Abstract: Provided herein are mycelium materials and methods for production thereof. In some embodiments, a mycelium material includes: a cultivated mycelium material including one or more masses of branching hyphae, wherein the one or more masses of branching hyphae may be disrupted or pressed and/or a bonding agent may be combined with the cultivated mycelium material. Methods of producing a mycelium material are also provided.

Abstract: This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.

Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to at least 2 distinct secretion signals.

Abstract: A method for manufacturing a garment includes gathering customer data from a plurality of customers, the customer data being associated with respective ones of a plurality of garment orders and including customer information regarding at least one customizable garment parameter and generating a schedule for producing a plurality of customized garments including analyzing the at least one customizable garment parameter for the at least some of the plurality of garment orders. The method further includes manufacturing the plurality of customized garments according to the schedule.

Abstract: Provided herein are mycelium materials and methods for production thereof. In some embodiments, a mycelium material includes: a cultivated mycelium material including one or more masses of branching hyphae, wherein the one or more masses of branching hyphae may be disrupted or pressed and/or a bonding agent may be combined with the cultivated mycelium material. Methods of producing a mycelium material are also provided.

Abstract: A method for manufacturing a knitted garment includes obtaining customer data regarding at least one customizable garment parameter and applying at least one sequencing module to the customer data to generate a pattern for a customized garment according to the at least one customizable garment parameter. The at least one sequencing module includes a radial symmetry module that includes applying a first transfer sequence to a first section of stitches including executing an underlapping transfer of stitches from successive pairs of needles in the second bed to successive single needles in the first bed, respectively, and applying a second transfer sequence to a second section of stitches including executing an overlapping transfer of stitches from successive pairs of needles in the second bed to successive single needles in the first bed, respectively.

Abstract: Disclosed herein are modified strains for reducing degradation of recombinantly expressed products secreted from a host organism and methods of using the modified strains. In some embodiments, to attenuate a protease activity in Pichia pastoris, the genes encoding enzymes the degrade proteases are inactivated or mutated to reduce or eliminate activity. In preferred strains, the protease activity of proteases encoded by PAS_chr4_0584 (YPS1-1) and PAS_chr3_1157 (YPS1-2) (e.g., polypeptides comprising SEQ ID NO: 66 and 67) is attenuated.

Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.