Patents Assigned to Bolt Threads, Inc.
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Patent number: 12268275Abstract: Disclosed herein are improved cross-linked resilin compositions and methods of making these improved compositions. These include new methods of cross-linking resilin compositions, use of polar nonaqueous solvents for adjusting material properties of cross-linked resilin solid compositions, and resilin foam compositions and methods of making the same.Type: GrantFiled: July 18, 2019Date of Patent: April 8, 2025Assignee: BOLT THREADS, INC.Inventors: Matthew Jordan Smith, Michael Eun-Suk Lee, Mitchell Joseph Heinrich, Paul James Jehlen
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Patent number: 12209331Abstract: Provided herein are scalable methods of processing wet-spun fiber comprising recombinant spider silk polypeptides to generate a three-dimensional crystalline lattice of beta-sheet structures in the fiber.Type: GrantFiled: November 16, 2021Date of Patent: January 28, 2025Assignee: BOLT THREADS, INC.Inventors: Amir Ahmad Bakhtiary Davijani, Steven Tom, Maxime Boulet-Audet, Paul Andre Guerette, Lindsay Wray, Nicole Elizabeth Subler, Zoya Nasir Zulfiqar, Hua Li, Jessica Wang
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Patent number: 12122810Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to at least 2 distinct secretion signals.Type: GrantFiled: May 23, 2022Date of Patent: October 22, 2024Assignee: BOLT THREADS, INC.Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce
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Patent number: 11891514Abstract: Provided herein are mycelium materials and methods for production thereof. In some embodiments, a mycelium material includes: a cultivated mycelium material including one or more masses of branching hyphae, wherein the one or more masses of branching hyphae may be disrupted or pressed and/or a bonding agent may be combined with the cultivated mycelium material. Methods of producing a mycelium material are also provided.Type: GrantFiled: April 9, 2021Date of Patent: February 6, 2024Assignee: BOLT THREADS, INC.Inventors: Matthew Jordan Smith, Julian Goldman, Maxime Boulet-Audet, Steven Joseph Tom, Hua Li, Tyler John Hurlburt
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Patent number: 11858971Abstract: This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.Type: GrantFiled: March 31, 2021Date of Patent: January 2, 2024Assignee: BOLT THREADS, INC.Inventors: Joshua Kittleson, Michael Lee, David N. Breslauer, Daniel M. Widmaier
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Patent number: 11725030Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the ?-mating factor (?MF) of Saccharomyces cerevisiae and a non-?MF signal peptide.Type: GrantFiled: March 15, 2022Date of Patent: August 15, 2023Assignee: BOLT THREADS, INC.Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce, David N. Breslauer, Daniel M. Widmaier
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Patent number: 11634729Abstract: Disclosed herein are modified strains for improving secretion of recombinantly expressed products secreted from a host organism with improved growth and productivity characteristics, as well as methods of using the modified strains.Type: GrantFiled: May 17, 2019Date of Patent: April 25, 2023Assignee: BOLT THREADS, INC.Inventor: Thomas Stevens
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Patent number: 11519109Abstract: A method for manufacturing a garment includes gathering customer data from a plurality of customers, the customer data being associated with respective ones of a plurality of garment orders and including customer information regarding at least one customizable garment parameter and generating a schedule for producing a plurality of customized garments including analyzing the at least one customizable garment parameter for the at least some of the plurality of garment orders. The method further includes manufacturing the plurality of customized garments according to the schedule.Type: GrantFiled: August 3, 2020Date of Patent: December 6, 2022Assignee: BOLT THREADS, INC.Inventors: Myrrhia Rae Resneck, Mitchell Joseph Heinrich
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Patent number: 11505654Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.Type: GrantFiled: June 22, 2018Date of Patent: November 22, 2022Assignee: BOLT THREADS, INC.Inventors: Daniel M. Widmaier, David N. Breslauer, Joshua Kittleson, Brendan Turner, Lindsay Wray
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Patent number: 11447532Abstract: The present disclosure provides improved long uniform recombinant protein fibers with desirable physical traits. The present disclosure also provides compositions derived from the long uniform recombinant protein fibers.Type: GrantFiled: September 14, 2017Date of Patent: September 20, 2022Assignee: BOLT THREADS, INC.Inventors: David Breslauer, Joshua Kittleson, Loren Perelman, Lindsay Wray
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Patent number: 11306127Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the ?-mating factor (?MF) of Saccharomyces cerevisiae and a non-?MF signal peptide.Type: GrantFiled: March 13, 2018Date of Patent: April 19, 2022Assignee: Bolt Threads, Inc.Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce, David N. Breslauer, Daniel M. Widmaier
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Patent number: 11214785Abstract: Disclosed herein are modified strains for reducing degradation of recombinantly expressed products secreted from a host organism and methods of using the modified strains. In some embodiments, to attenuate a protease activity in Pichia pastoris, the genes encoding enzymes the degrade proteases are inactivated or mutated to reduce or eliminate activity. In preferred strains, the protease activity of proteases encoded by PAS_chr4_0584 (YPS1-1) and PAS_chr3_1157 (YPS1-2) (e.g., polypeptides comprising SEQ ID NO: 66 and 67) is attenuated.Type: GrantFiled: April 7, 2020Date of Patent: January 4, 2022Assignee: Bolt Threads, Inc.Inventors: Matthew Scott Gamboa, Joshua Tyler Kittleson
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Patent number: 11208736Abstract: Provided herein are scalable methods of processing wet-spun fiber comprising recombinant spider silk polypeptides to generate a three-dimensional crystalline lattice of beta-sheet structures in the fiber.Type: GrantFiled: September 25, 2018Date of Patent: December 28, 2021Assignee: Bolt Threads, Inc.Inventors: Amir Ahmad Bakhtiary Davijani, Steven Tom, Maxime Boulet-Audet, Paul Andre Guerette, Lindsay Wray, Nicole Elizabeth Subler, Zoya Nasir Zulfiqar, Hua Li, Jessica Wang
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Patent number: 11192982Abstract: The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.Type: GrantFiled: August 30, 2019Date of Patent: December 7, 2021Assignee: Bolt Threads, Inc.Inventors: Daniel M. Widmaier, David N. Breslauer, Joshua Kittleson, Brendan Turner, Lindsay Wray
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Patent number: 11178934Abstract: An article of footwear includes an upper and a midsole coupled with the upper. The midsole includes at least a portion of a solid resilin material comprising a cross-linked recombinant resilin and a polar nonaqueous solvent.Type: GrantFiled: July 16, 2019Date of Patent: November 23, 2021Assignee: Bolt Threads Inc.Inventors: Matthew Jordan Smith, Michael Eun-Suk Lee, Mitchell Joseph Heinrich, Paul James Jehlen
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Patent number: 11015059Abstract: Provided herein are mycelium materials and methods for production thereof. In some embodiments, a mycelium material includes: a cultivated mycelium material including one or more masses of branching hyphae, wherein the one or more masses of branching hyphae may be disrupted or pressed and/or a bonding agent may be combined with the cultivated mycelium material. Methods of producing a mycelium material are also provided.Type: GrantFiled: July 30, 2020Date of Patent: May 25, 2021Assignee: Bolt Threads, Inc.Inventors: Matthew Jordan Smith, Julian Goldman, Maxime Boulet-Audet, Steven Joseph Tom, Hua Li, Tyler John Hurlburt
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Patent number: 10988515Abstract: This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.Type: GrantFiled: January 16, 2018Date of Patent: April 27, 2021Assignee: Bolt Threads, Inc.Inventors: Joshua Kittleson, Michael Lee, David N. Breslauer, Daniel M. Widmaier
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Patent number: 10906947Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to at least 2 distinct secretion signals.Type: GrantFiled: March 13, 2018Date of Patent: February 2, 2021Assignee: Bolt Threads, Inc.Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce
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Publication number: 20210017681Abstract: A method for manufacturing a garment includes gathering customer data from a plurality of customers, the customer data being associated with respective ones of a plurality of garment orders and including customer information regarding at least one customizable garment parameter and generating a schedule for producing a plurality of customized garments including analyzing the at least one customizable garment parameter for the at least some of the plurality of garment orders. The method further includes manufacturing the plurality of customized garments according to the schedule.Type: ApplicationFiled: August 3, 2020Publication date: January 21, 2021Applicant: Bolt Threads Inc.Inventors: Myrrhia Rae Resneck, Mitchell Joseph Heinrich
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Publication number: 20200392341Abstract: Provided herein are mycelium materials and methods for production thereof. In some embodiments, a mycelium material includes: a cultivated mycelium material including one or more masses of branching hyphae, wherein the one or more masses of branching hyphae may be disrupted or pressed and/or a bonding agent may be combined with the cultivated mycelium material. Methods of producing a mycelium material are also provided.Type: ApplicationFiled: July 30, 2020Publication date: December 17, 2020Applicant: Bolt Threads, Inc.Inventors: Matthew Jordan Smith, Julian Goldman, Maxime Boulet-Audet, Steven Joseph Tom, Hua Li, Tyler John Hurlburt