Abstract: The invention provides a differentiation protocol to generate retinal pigmented epithelial cells (RPECs) for the in vitro differentiation of mammalian pluripotent stem cells to mature, functional retinal pigment epithelial cells (RPEC). A culture system is described that uses defined medium comprising xeno-free optimal combinations of small molecule agonists and antagonists where: (a) pluripotent stem cells are cultured in the absence of feeder cells, serum, or conditioned medium; (b) defined culture conditions is used to obtain an eye-field cell (EFC) population; (c) whereby EFC are subsequently differentiated to RPEC when exposed to culture medium containing small molecule agonists thus producing highly homogenous RPEC; and (d) cultured under optimal conditions until maturation, through stimulation via activin-like signalling through addition of either cyclic AMP or cyclic AMP inducers to produce complete monolayer formation within 21+7 days.

Abstract: The present invention relates to a customized composition comprising three-dimensional (3D) nanofiber webbing. The present invention further relates to the process of producing the composition comprising 3D nanofiber webbing and uses thereof such as treatment of age-related macular degeneration or regeneration/repair of tissue.

Abstract: The present invention relates to a customized composition comprising three-dimensional (3D) nanofiber webbing. The present invention further relates to the process of producing the composition comprising 3D nanofiber webbing and uses thereof such as treatment of age-related macular degeneration or regeneration/repair of tissue.

Abstract: A method and system for classifying tissue suspected of being abnormal as being malignant or benign. The method includes generating a set of selection features, performing statistical applications to generate additional selection features, generating a feature vector for the abnormal tissue, feeding the feature vector into a neural network, and obtaining a result from the neural network as to whether the abnormal tissue is malignant or benign. The method and system may be used for determining the presence of breast cancer.