Abstract: Embodied herein are methods of reprogramming somatic cells or tissue stem cells to a more multipotent state or even a pluripotent state, the methods do not involve gene transfer of master transcription factor genes/proteins. The methods are also useful for rapid and efficient cloning of induced pluripotent stem cells after gene transfer of master transcription factor genes/proteins.
Type:
Grant
Filed:
December 3, 2010
Date of Patent:
June 24, 2014
Assignee:
Boston Biomedical Research Institute, Inc.
Abstract: This invention provides methods of determining the number and percent of tissue specific stem cells (TSSCs) in a sample of cells, a population of cells or a sample of tissue. The methods rely on detecting the pattern-specific asymmetric localization of asymmetric self-renewal associated (ASRA) proteins or cell cycle specific proteins (CSSP) in cell undergoing asymmetrical self-renewal, which is a characteristic of TSSCs. The methods can be applied to any situations in which the percent of TSSC is desired such as laboratory research on adult stem cells, in drug development tests, prognostic indicator and therapeutic index, as a diagnostic and prognostic indicator and in monitoring TSSC expansion, e.g., in cell manufacturing processes.
Type:
Application
Filed:
December 3, 2010
Publication date:
January 3, 2013
Applicant:
BOSTON BIOMEDICAL RESEARCH INSTITUTE, INC.
Abstract: Embodied herein are methods of reprogramming somatic cells or tissue stem cells to a more multipotent state or even a pluripotent state, the methods do not involve gene transfer of master transcription factor genes/proteins. The methods are also useful for rapid and efficient cloning of induced pluripotent stem cells after gene transfer of master transcription factor genes/proteins.
Type:
Application
Filed:
December 3, 2010
Publication date:
November 22, 2012
Applicant:
BOSTON BIOMEDICAL RESEARCH INSTITUTE, INC.
Abstract: A homogenous sample of identical bispecific antibody determinants, each determinant being composed of two L-H half-molecules linked by disulfide bonds, each L-H half-molecule being specific for a different antigenic determinant and including at least the F(ab') portion of a monoclonal IgG antibody. The bispecific antibody determinants are useful, e.g., in the formation of multilamellar assemblies and in enzymatic assays.
Type:
Grant
Filed:
June 17, 1992
Date of Patent:
June 4, 1996
Assignee:
Boston Biomedical Research Institute, Inc.
Abstract: A homogenous sample of identical bispecific antibody determinants, each determinant being composed of two L-H half-molecules linked by disulfide bonds, each L-H half-molecule being specific for a different antigenic determinant and including at least the F(ab') portion of a monoclonal IgG antibody. The bispecific antibody determinants are useful, e.g., in the formation of multilamellar assemblies and in enzymatic assays.
Type:
Grant
Filed:
December 5, 1990
Date of Patent:
March 8, 1994
Assignee:
Boston Biomedical Research Institute, Inc.
Abstract: A homogenous sample of identical bispecific antibody determinants, each determinant being composed of two L-H half-molecules linked by disulfide bonds, each L-H half-molecule being specific for a different antigenic determinant and including at least the F(ab') portion of a monoclonal IgG antibody. The bispecific antibody determinants are useful, e.g., in the formation of multilamellar assemblies and enzyme electrodes.
Type:
Grant
Filed:
December 21, 1981
Date of Patent:
April 24, 1984
Assignee:
Boston Biomedical Research Institute, Inc.