Abstract: Related are a CSPG4-targeting humanized chimeric antigen receptor and applications thereof, comprising a humanized anti-CSPG4 binding domain, a hinge region, a transmembrane domain, and a signal transduction domain. The anti-CSPG4 binding domain comprises an anti-CSPG4 antibody or antigen binding part. Related are an immune effector cell expressing the CSPG4-targeting humanized chimeric antigen receptor and applications of the cell.