Abstract: A blood sample collection apparatus includes a removable plug adapted to fit within the open end of a blood collection tube. A vacuum tube extends through the plug for evacuating air from the collection tube. A pickup tube protrudes outwardly from the plug for supplying blood to a drop tube extending downwardly from the plug with its lower end positioned within the collection tube substantially below the lower end of the vacuum tube. A primary filter is sealed against the underside of the plug to filter the air drawn through the vacuum tube by a portable hand-held vacuum source. The primary filter is a hydrophobic microporous membrane operative to allow airflow therethrough but to block liquid flow therethrough. A secondary filter is operatively interposed between the vacuum tube and he vacuum source for increased safety. Installation of the secondary filter bypasses a bleeder port which renders the apparatus inoperative in the absence of the secondary filter.