Abstract: Disclosed is a method for manufacturing an electrode pattern of a disposable electrochemical sensor strip. The method comprises steps of preparing a nonconductive substrate; forming a mask film with an inverse pattern on at least one side of the nonconductive substrate, said mask film is made of water soluble material or solvent soluble material; forming a metal film both on the mask film and the nonconductive substrate; and washing out, by water, solvent, or water solution, the mask film and the metal film which is on the mask film, so as to form a metal electrode with the electrode pattern on at least one side of the substrate of the disposable electrochemical sensor strip.