Abstract: A sorting apparatus and method for fractionating and simultaneously viewing individual microstructures, such as free cells, viruses, macromolecules, or minute particles in a fluid medium. The sorting apparatus is composed of a substrate having a receptacle located therein, the receptacle having sidewalls and a floor. An array of obstacles is positioned within the receptacle with the obstacles upstanding from the floor of the receptacle. A transparent cover overlies the array of obstacles to cover the receptacle and afford visual observation of migration of the microstructures exclusively through the array of obstacles. Electrodes may be positioned within the receptacle to generate an electric field in the fluid medium in the receptacle in order to induce the migration of the microstructures. Migration of the microstructures may also occur, for example, by a hydrodynamic field, an optical field, a magnetic field, or a gravity field applied to the receptacle.

Abstract: A cloned gene or fragment thereof encodes antigenic proteins that bind with a monoclonal or polyvalent antibody that is directed against an antigenic protein of avian coccidia.

Abstract: A gradient coil specifically designed to enhance the performance of Magnetic Resonance (MR) imaging and spectroscopy. In particular, the construction of gradient coils for use with resonant gradient power supplies to provide the necessary performance (large electromagnetic fields that can activated and deactivated rapidly) required in MR systems. These gradient coils require considerably less power than those presently employed for comparable performance and provide considerably more performance with existing power supplies. These coils are characterized by having a higher inductance than existing gradients of comparable size. Examples are given for the use of the invention in providing linear axial gradient field via a standard Maxwell gradient coil, with comparisons with existing coils.

Abstract: This invention relates to novel recombinant antigenic proteins of avian coccidiosis, and fragments thereof containing antigenic determinants, and to the genes that encode the antigenic peptides. This invention also relates to vaccines made using the novel antigenic proteins of avian coccidiosis and to methods of immunizing chickens against avian coccidia.

Abstract: Fluorescent and/or chromogenic reagents in which a phthalocyanine derivative is monomerically conjugated with an antigen, antibody, oligonucleotide, or nucleic acid. Methods are presented in in which greater than 90% of the phthalocyanine dyes are monomeric when conjugated. This greatly enhances their performance as detectable markers in immunoassays, nucleic acid probe assays, immunoblotting, hybridization assays, microscopy, imaging, flow cytometry, DNA sequencing, and photodynamic therapy. For use as fluorophores, the free base phthalocyanine may or may not be metallated. Metals for fluorescent phthalocyanine include aluminum, silicon, phosphorus, gallium, germanium, cadmium, scandium, magnesium, tin, and zinc. For use as chromogens, the phthalocyanine may or may not be metallated. For use in aqueous solution, the phthalocyanine macrocycle should be derivatized with water-solubilizing substituents such as sulfonic acid, phosphate, phosphonate, hydroxy, phenoxy, amino, ammonium, or pyridinium groups.

Abstract: A lifting body moves relative to a fluid, thereby creating a vortex field in the fluid downstream of the lifting body. The lifting body has a predetermined lift distribution along the length thereof which enhances the velocity component of the fluid flow directed outwardly from the centroid of the vortex field to reduce the strength of trailing vortices. In a preferred embodiment, the lifting body is a wing with a perturbation proximate to the tip end of the wing planform trailing edge.

Abstract: This invention relates to novel recombinant antigenic proteins of avian coccidiosis, and fragments thereof containing antigenic determinants, and to the genes that encode the antigenic peptides. This invention also relates to vaccines made using the novel antigenic proteins of avian coccidiosis and to methods of immunizing chickens against avian coccidia.

Abstract: A defibrillator and demand pacer catheter is defined by a flexible, electrically nonconductive probe having an electrically conductive pathway longitudinally disposed therein. Attached to one end of the probe is a defibrillator electrode capable of anchoring the probe to the septum of a heart and of transmitting from said conductive pathway directly to the interior of the septum a portion of an electrical defibrillation pulse sufficient to defibrillate the heart. The defibrillation pulse is delivered in such a manner so as to avoid injuring the heart tissue immediately adjacent to the defibrillator electrode. In the preferred embodiment, the defibrillator electrode is helical; however, it is also envisioned as being a lance. Alternatively, the catheter further comprises a ground electrode, a demand pacer electrode, and a supplemental defibrillator electrode attached to the probe.

Abstract: The boundary layer of a fluid travelling in a mean-flow direction relative to a surface of a wall of a body is controlled by generating in the fluid a magnetic field B having flux lines along the surface of the wall and an electric current density J traversing the magnetic flux lines in the fluid to form a control region. The magnetic field B and the electric current density J create in the control region a force J.times.B that can stabilize or destabilize flow in the boundary layer. A plurality of such control regions can be arranged in an two-dimensional array of control tiles that are periodically actuated in a controlled, predetermined pattern at a critical frequency that provides boundary layer control over a given area.

Abstract: A sorting apparatus and method for fractionating and simultaneously viewing individual microstructures, such as free cells, viruses, macromolecules, or minute particles in a fluid medium. The sorting apparatus is composed of a substrate having a receptacle located therein, the receptacle having sidewalls and a floor. An array of obstacles is positioned within the receptacle with the obstacles upstanding from the floor of the receptacle. A transparent cover overlies the array of obstacles to cover the receptacle and afford visual observation of migration of the microstructures exclusively through the array of obstacles. Electrodes may be positioned within the receptacle to generate an electric field in the fluid medium in the receptacle in order to induce the migration of the microstructures. Migration of the microstructures may also occur, for example, by a hydrodynamic field, an optical field, a magnetic field, or a gravity field applied to the receptacle.

Abstract: An inta-oral device for viewing the oral cavity comprises a handle having a tube extending therefrom for insertion into the oral cavity to be viewed; an optical system within the tube; a video camera within the handle and illuminating means for illuminating the cavity; and a removable, rigid, sterilizable sheath completely enclosing the tube and illuminating means to prevent their exposure to the oral cavity. The optical means provides a non-reversed image to appear on a video screen.

Abstract: An anti-coccidial vaccine is provided which contains a recombinant peptide with novel epitopes. The recombinant peptide has an amino-terminal amino acid sequence that is unique among known Eimeria antigens. Recombinant vectors encoding the novel peptide antigen are deposited under ATCC Accession No. 68450, and ATCC Accession No. 68537.

Abstract: A defibrillator and demand pacer catheter is defined by a flexible, electrically nonconductive probe having an electrically conductive pathway longitudinally disposed therein. Attached to one end of the probe is a defibrillator electrode capable of anchoring the probe to the septum of a heart and of transmitting from said conductive pathway directly to the interior of the septum a portion of an electrical defibrillation pulse sufficient to defibrillate the heart. The defibrillation pulse is delivered in such a manner so as to avoid injuring the heart tissue immediately adjacent to the defibrillator electrode. In the preferred embodiment, the defibrillator electrode is helical; however, it is also envisioned as being a lance. Alternatively, the catheter further comprises a ground electrode, a demand pacer electrode, and a supplemental defibrillator electrode attached to the probe.

Abstract: The boundary layer of a fluid travelling in a mean-flow direction relative to a surface of a wall of a body is controlled by generating in a near-wall region of the flow a magnetic field B having flux lines parallel to the surface of the wall and an electric current density J traversing the magnetic flux lines in the fluid. An electrolyte or other conductivity-enhancing material is introduced into the flow to provide an electrical conductivity gradient in the near-wall region. The magnetic field B and the electric current density J create in the fluid a force J.times.B having a component normal to the surface of the wall that because of the increased conductivity gradient near the surface can stabilize or destabilize flow in the boundary layer. Numerous aspects of the fluid flow and its interaction with the body can thus be controlled.

Abstract: A primary gradient coil and a screen coil for use with the primary coil are constructed to create a substantially null field adjacent to the screen coil and a substantially distortion free linear gradient field adjacent to the gradient coil. A method is disclosed for generating the coils by considering the screen coil to be of infinite length, deriving a Fourier transform of the axial and other components of the field in which the transform is the sum of the permissible harmonic modes on the coil surface in which a coefficient C.sub.n is derived representing the nth harmonic. A perfect screen coil is created and the number of terms C.sub.n selected using least squares optimization until there is no longer a significant change in the resulting field. Examples are given for creating unscreened, screened, linear, axial and transverse gradient field coils.

Abstract: A polyhedral approximation of an Earth globe or other non-planar surface and a projection of such non-planar surface to a two-dimensional form with a practical minimum of distortion of principal and secondary features is disclosed. Vertices are selected on the non-planar surface to be mapped so as to define identifiable panels which are suitable for individual study because of their incorporation of at least one of the principal features wholly within one of the panels. Vertices are selected so that the panels are individually shaped and sized to accommodate at least a majority of at least one of the principal features.

Abstract: Red-shifted, water-soluble, fluorescent, monomerically-tetherable derivatives having the formula: ##STR1## wherein, M represents either H.sub.2 or is selected from among the following metals: aluminum, silicon, phosphorus, gallium, germanium, cadmium, scandium, magnesium, tin, and zinc. Each R.sub.1 is independently selected from --XYW, --YW, and --W. X represents either a carbon, or heteroatom selected from among oxygen, nitrogen, sulfur, phosphorus, silicon, and selenium; Y represents a linking group; and W represents a water solubilizing group. The substituent R.sub.2 is selected from among --A, --Y'A, --XA, and --XY'A, where A denotes a biological entity such as an antibody, antibody fragment, nucleotide, nucleic acid probe, antigen, oligonucleotide, deoxynucleotide, dideoxynucleotide, avidin, streptavidin or membrane probe, or R.sub.2 is a reactive or activatable group suitable for conjugating to a biological entity.

Abstract: Sperm are encapsulated in a nontoxic polymer which is freely flowing at body temperature and a gel or solid at temperatures of storage and transfer. On delivery to the reproductive tract, the polymer microcapsule liquifies and the sperm are released.

Abstract: An integrated, miniaturized, imaging assembly, comprising microscope size and arrangement, video camera lenses having the objective lens in a mount that is preferably a blackened sphere and that is pivotally mounted in a supporting structure rearwardly of but adjacent to the objective lens, so the forward end of the lens mount can be placed within a concave dome of unusually small and shallow size with the remainder of the imaging assembly outside of and behind the concavity of such viewing dome, and can be tilted and panned to sweep over a broad viewing area with minimum intrusion thereinto. The imaging assembly includes an electronic image-acquisition device, in line with the optical axis of the lenses, and electronic camera circuitry preferably remote therefrom. An easily movable, tripod type of mount for the imaging assembly is adapted to rest on ceiling or other structure in which the viewing dome is mounted.