Abstract: A replaceable cartridge column for use in high pressure liquid chromatograph pumping systems including a solid tube having a small bore therethrough carrying a packing material. The tube and terminated by a recess at each end which extends from the rim at the end of the tube inwardly and in communication with the bore. A plastic seal plug is disposed in the recess at each end to extend slightly beyond excess the length of the tube when so disposed and carries at its outer end a coaxial frit filter. The recess is provided with a flat bottom and the plastic plug has a corresponding bottom constructed to be positioned in face-to-face sealing opposition to fluid leakage and having further a flat sealing surface at its other end to engage a sealing surface of an end fitting of the liquid chromatographic pumping system and to provide a first, primary seal therewith independent of the rim of the column tube.

Abstract: A coupling for high pressure liquid chromatographic columns of any length, individually, or by union of two columns together, in which an end fitting carries a secondary seal together with internal means for retaining the seal in place. The end fitting is designed as a unitary assembly wherein the secondary seal is secured into the end fitting as a unitary assembly of parts without the use of screw threaded members. A special screw and nut configuration of end fittings permits the same design to be used for columns of extremely short lengths.

Abstract: A coupler or union for passing fluid under pressure from a column or tube in which the tube is received within a chamber in fluid communication through the floor within said coupler and forming a zero dead volume seal therewith, an annular recess in the coupler or union surrounding the tube or column and containing a balanced hydraulic seal in contact with the recess wall and tube or column and opening in communication toward said dead volume seal so that leakage from the latter under high pressure fills and activates the balanced hydraulic seal to the line pressure. The coupler or union is particularly applicable for high pressure liquid chromatograph column connections.

Abstract: Plastic seal plugs insert into each end of an elution column tube and are provided with flat gasket portions extending laterally to cover each tube end, a spring loaded hand-tightenable nut drives an end fitting from each end of a column holder extending alomst the length of the column to put the gasket portions and the tube in compression and create a seal between the end fitting, tube, at interposed gasket, the latter being entirely contained against cold flow or deformation by a close fitting recess in the end fitting which entirely captures the plastic gasket and tube end.

Abstract: Plastic seal plugs insert into each end of an elution column tube and are provided with flat gasket portions extending laterally to cover each tube end, a spring loaded hand-tightenable nut drives an end fitting from each end of a column holder extending alomst the length of the column to put the gasket portions and the tube in compression and create a seal between the end fitting, tube, at interposed gasket, the latter being entirely contained against cold flow or deformation by a close fitting recess in the end fitting which entirely captures the plastic gasket and tube end.