Abstract: The present invention relates to a method for rapidly detecting quinolone-resistant Salmonella spp. The invention also relates to the oligonucleotide primers and probes utilized in the method, which can differentiate mutant strains having one or two single point mutations in gyrA gene or single point mutation in parC gene from wild type strains, respectively. Said primers and probes can be effectively used for detection of nalidixic acid-resistant and/or ciprofloxacin-resistant Salmonella strains.
Type:
Application
Filed:
October 18, 2006
Publication date:
February 8, 2007
Applicant:
Bureau of Food and Drug Analysis, Department of Health, Executive Yuan
Inventors:
Shu-Jean Tsai, Shu-Wan Wang, Daniel Shih
Abstract: The present invention relates to a reference plasmid for use in specifically detecting the meat of pig, cattle, sheep, deer, horse and kangaroo. The reference plasmid can be used in rapidly identifying the species of meat and can be used in testing commercial meat products.
Type:
Application
Filed:
July 5, 2005
Publication date:
January 11, 2007
Applicant:
Bureau of Food and Drug Analysis, Department of Health, Executive Yuan
Abstract: The present invention relates to a method for testing the presence or absence of an animal-derived ingredient(s) in food, which comprises the step of (i) detecting whether a DNA fragment highly reserved among animals is present, and optionally the step of (ii) detecting whether a DNA fragment highly reserved among mammals and poultry is present, and optionally the step of (iii) detecting whether a DNA fragment highly reserved in a specific animal species is present.
Type:
Application
Filed:
September 23, 2005
Publication date:
May 11, 2006
Applicant:
Bureau of Food and Drug Analysis, Department of Health, Executive Yuan
Abstract: The present invention relates to a method for rapidly detecting quinolone-resistant Salmonella spp. The invention also relates to the oligonucleotide primers and probes utilized in the method, which can differentiate mutant strains having one or two single point mutations in gyrA gene or single point mutation in parC gene from wild type strains, respectively. Said primers and probes can be effectively used for detection of nalidixic acid-resistant and/or ciprofloxacin-resistant Salmonella strains.
Type:
Application
Filed:
December 20, 2004
Publication date:
February 16, 2006
Applicant:
Bureau of Food and Drug Analysis, Department of Health, Executive Yuan
Inventors:
Shu-Jean Tsai, Shu-Wan Wang, Daniel Shih