Abstract: Provided herein are processes and microorganisms which utilize both protein hydrolysates and carbohydrates from biomass feedstocks to produce renewable hydrocarbon compositions. Advantages of the disclosed methods may be recognized in fuel blends comprising such hydrocarbon compositions.
Type:
Grant
Filed:
September 20, 2013
Date of Patent:
December 12, 2017
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Larry Cameron Anthony, Tyler T. Ames, Keith H. Burlew
Abstract: Provided are methods for removing one or more components from a butanol based composition. The methods comprise providing a butanol based composition comprising one or more components, targeting at least one component or a combination thereof for reduction, and processing said butanol based composition such that the at least one targeted component is substantially removed. The butanol based composition can, for example, be bio-produced.
Type:
Grant
Filed:
July 26, 2013
Date of Patent:
November 7, 2017
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Cecilia Cheng, Ian David Dobson, Andrew John Hogben, Andrew Richard Lucy, John Glenn Sunley, Leslie William Bolton
Abstract: Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.
Type:
Grant
Filed:
March 23, 2012
Date of Patent:
October 17, 2017
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Larry Cameron Anthony, Hongxian He, Lixuan Lisa Huang, Daniel P. Okeefe, Arthur Leo Kruckeberg, Yougen Li, Lori Ann Maggio-Hall, Jessica McElvain, Mark J. Nelson, Ranjan Patnaik, Steven Cary Rothman
Abstract: Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products. Host cells provided herein comprise a pyruvate-utilizing pathway and a competitive growth advantage over other microorganisms in solution.
Abstract: The invention relates to recombinant host cells having at least one integrated polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway, e.g., pyruvate to acetolactate conversion. The invention also relates to methods of increasing the biosynthetic production of isobutanol, 2,3-butanediol, 2-butanol or 2-butanone using such host cells.
Type:
Grant
Filed:
January 8, 2016
Date of Patent:
September 19, 2017
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Larry Cameron Anthony, Lori Ann Maggio-Hall
Abstract: The present invention relates to processes for recovering butanol produced in a fermentative process using, for example, an ethanol production plant which has been reversibly-retrofitted for butanol production, that is, the ethanol production plant may be converted for butanol production, but can also revert to an ethanol production. The present invention also relates to processes for recovering butanol produced in a fermentative process in a butanol production plant that may be converted to ethanol production plant.
Type:
Grant
Filed:
May 3, 2013
Date of Patent:
August 15, 2017
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Steven Christopher Barr, Robert W. Sylvester, Joseph J. Zaher
Abstract: The invention relates to the fields of industrial microbiology and alcohol production. More specifically, the invention relates to improved production of butanol isomers by recombinant microorganisms containing an engineered butanol pathway and disrupted activity of the genes in pathways for the production of by-products during the fermentation when the microorganisms are grown in a fermentation medium containing acetate. In embodiments, recombinant microorganisms have an increased growth rate in a fermentation medium containing acetate as a C2 supplement.
Abstract: Provided herein are methods for recovering butanol from a fermentation medium. The methods comprise providing a fermentation medium comprising butanol, water, and a recombinant microorganism comprising a butanol biosynthetic pathway, wherein the recombinant microorganism produces butanol; contacting the fermentation medium with a water immiscible organic extractant composition comprising a dry solvent to form a butanol-containing organic phase and an aqueous phase; and recovering the butanol from the butanol-containing organic phase.
Abstract: A method and system for efficiently producing a fermentative product alcohol such as butanol utilizing in situ product extraction are provided. The efficiency is obtained through separating undissolved solids after liquefying a given feedstock to create a feedstock and prior to fermentation, for example, through centrifugation. Removal of the undissolved solids avoids problems associated with having the undissolved solids present during in situ production extraction, and thereby increases the efficiency of the alcohol production.
Type:
Grant
Filed:
May 1, 2013
Date of Patent:
June 6, 2017
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Brian Michael Roesch, Keith H. Burlew, John W. Hallam, David J. Lowe, Joseph J. Zaher
Abstract: Provided herein are processes for producing an improved culture of cells comprising an engineered butanol biosynthetic pathway. The processes comprise (a) providing a cell culture of recombinant microorganisms comprising an engineered butanol biosynthetic pathway, wherein the engineered butanol biosynthetic pathway is minimal or not activated; and (b) growing the culture of recombinant microorganisms under adaptive conditions whereby pathway activation is increased to produce an improved cell culture and whereby the improved cell culture is capable of continuing to grow in fermentation.
Type:
Grant
Filed:
July 1, 2014
Date of Patent:
May 30, 2017
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Ritu Bhalla, Steven D. Doig, Kakasaheb Suresh Konde, Ranjan Patnaik
Abstract: A method for producing a gasoline blend having a high concentration of a butanol isomer and having good cold start and warm-up driveability performance.
Abstract: Dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
Type:
Grant
Filed:
December 27, 2013
Date of Patent:
May 16, 2017
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Lori Ann Maggio-Hall, Brian James Paul, Steven Cary Rothman, Rick W. Ye
Abstract: The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
Type:
Grant
Filed:
March 15, 2013
Date of Patent:
April 4, 2017
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Dennis Flint, Brian James Paul, Rick W. Ye
Abstract: The present invention relates to the fermentative production of alcohols including ethanol and butanol, and processes for improving alcohol fermentation employing in situ product removal methods.
Type:
Grant
Filed:
March 14, 2013
Date of Patent:
March 28, 2017
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Stephane Francois Bazzana, James Timothy Cronin, Michael Charles Grady, David J. Lowe, Brian Michael Roesch, Joseph J. Zaher, Raymond Richard Zolandz
Abstract: The invention relates to the development of microorganisms capable of producing fermentation products via an engineered pathway in the microorganisms. The invention also relates to microorganisms with improved cell viability and methods to improve cell viability and cell productivity of a microorganism.
Abstract: Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
Abstract: The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.
Type:
Grant
Filed:
July 18, 2014
Date of Patent:
January 24, 2017
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Michael Dauner, Lori Ann Maggio-Hall, Jean-Francois Tomb
Abstract: The present invention relates to processes and systems for the production of fermentative products such as ethanol and butanol. The present invention also provides methods for separating feed stream components for improved biomass processing productivity.
Type:
Grant
Filed:
March 11, 2014
Date of Patent:
December 20, 2016
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Benjamin Fuchs, Brian Michael Roesch, Mathias E. Stolarski, James Gregory Wood
Abstract: The invention relates to a method for producing butanol through microbial fermentation, in which the butanol product is removed by extraction into a water immiscible organic extractant during the fermentation. The invention also relates to a method for producing butanol through microbial fermentation, in which the butanol product is removed during the fermentation by extraction into a water-immiscible extractant composition. The invention further relates to compositions comprising a solution of butanol in a water immiscible organic extractant composition.
Type:
Grant
Filed:
March 14, 2014
Date of Patent:
December 13, 2016
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Thomas Basham, Leslie William Bolton, Ian David Dobson, Sarah Richardson Hanson, Aidan Hurley, Karen Kustedjo, Andrew Richard Lucy, Liang Song
Abstract: The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
Type:
Grant
Filed:
March 15, 2013
Date of Patent:
December 6, 2016
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Dennis Flint, Brian James Paul, Rick W. Ye