Abstract: Provided herein are methods for selecting and stimulating a plurality of cells in a sample of cells using column chromatography, and collecting the cells without using additional steps or reagents to facilitate detachment of the cells from the column. In some aspects, the methods provided herein reduce the time needed to generate a population of selected and stimulated cells useful for genetic engineering, and ultimately, cell therapy, compared to existing methods. Also provided are articles of manufacture and apparatus thereof.
Type:
Grant
Filed:
October 30, 2019
Date of Patent:
January 13, 2026
Assignee:
C3S2 GMBH
Inventors:
Lothar Germeroth, Christian Stemberger, Mateusz Pawel Poltorak, Thomas Schmidt