Abstract: Embodiments of the present disclosure are directed to a plurality of nucleotide sequences encoding a first intein fused to at least a portion of a first transcription activator-like effector (TALE), a second nucleotide sequence encoding the first intein fused to at least a portion of a second TALE, and a third nucleotide sequence encoding a second intein fused to at least a portion of a rare-cutting nuclease.

Abstract: Example embodiments in accordance with the present disclosure are directed to methods comprising contacting a plant part with a nucleotide sequence encoding a gene that induces plant cell matrix (PCM) formation, and culturing the plant part under growth conditions to enhance PCM formation.

Abstract: Embodiments of the present disclosure are directed to a method that includes generating a chimeric plant by infecting a cutting of a host plant with Rhizobium rhizogenes carrying an expression construct, and screening new growth from the chimeric plant for gene edits resulting from genomic editing by the expression construct.

Abstract: Materials and methods are provided for making soybean varieties that have altered oil composition as a result of one or more mutation modulating the expression of a SACPD-C gene, a FATB-1A gene, or both the SACPD-C and FATB-1A genes. For example, a soybean plant, plant part, or plant cell producing an oil that has increased saturated fatty acid content as compared to oil produced from a corresponding soybean plant, plant part, or plant cell lacking the one or more mutations is provided.

Abstract: Embodiments of the present disclosure are directed to a method that includes contacting a population of plant cells with a messenger ribonucleic acid (mRNA) construct including a sequence encoding a rare-cutting endonuclease and a detectable label, wherein the rare-cutting endonuclease is configured to induce a mutation at a target genomic locus. The method further includes screening the population of plant cells for the detectable label to identify target plant cells that are genetically transformed with the mRNA construct.

Abstract: Embodiments of the present disclosure are directed to a method that includes treating a Cannabis plant, plant part, or plant cell with a solution including a chemical agent configured to modify a ploidy level of the Cannabis plant, plant part, or plant cell. The method further includes, in response to the treatment, modifying the ploidy level of the cannabis plant, plant part, or plant cell.

Abstract: TALEN compositions and methods of use are disclosed, which include using multiplexing compositions to create a targeted mutation in several genes at once, such as the FAD3 A/B/C genes, compositions to create a targeted mutation in a single gene, such as a gene encoding a FAD2 protein, and combinations thereof. The compositions and methods can provide gene-edited plants, plant parts, and plant cells that have improved characteristics compared to the corresponding unaltered plants, plant parts, or plant cells. For example, soybean plants, plant parts and plant cells that are capable of producing a seed with an oil having comparatively higher levels of oleic acid and lower levels of linoleic and linolenic acid than a corresponding seed lacking the targeted mutation are also provided.

Abstract: Example embodiments in accordance with the present disclosure are directed to methods comprising contacting a plant part with a nucleotide sequence encoding a gene that induces plant cell matrix (PCM) formation, and culturing the plant part under growth conditions to enhance PCM formation.