Patents Assigned to Cambia
  • Microbial B-glucuronidase genes, gene production and uses thereof
    Publication number: 20030157684
    Abstract: Genes encoding microbial &bgr;-glucuronidase and protein that is secreted and its uses are provided.
    Type: Application
    Filed: April 11, 2002
    Publication date: August 21, 2003
    Applicant: CAMBIA
    Inventors: Richard A. Jefferson, Rebecca L. Harcourt, Andrzej Kilian, Katherine Joanna Wilson, Paul Konrad Keese
  • Glucuronide repressors and uses thereof
    Publication number: 20030143709
    Abstract: Clones containing a sequence encoding a glucuronide repressor are described. The nucleotide and amino acid sequences of a repressor (gusR) are presented. A glucuronide repressor is used to control expression of a transgene, detect glucuronides in a sample, and isolate glucuronides from a sample, among other uses.
    Type: Application
    Filed: July 16, 2002
    Publication date: July 31, 2003
    Applicant: CAMBIA
    Inventors: Richard A. Jefferson, Katherine J. Wilson
  • Glucuronide repressors and uses thereof
    Patent number: 5879906
    Abstract: Clones containing a sequence encoding a glucuronide repressor are described. The nucleotide and amino acid sequences of a repressor (gusR) are presented. A glucuronide repressor is used to control expression of a transgene, detect glucuronides in a sample, and isolate glucuronides from a sample, among other uses.
    Type: Grant
    Filed: June 25, 1997
    Date of Patent: March 9, 1999
    Assignee: Cambia Biosystems LLC
    Inventors: Richard A. Jefferson, Katherine J. Wilson, Michael Leader
  • .beta.-glucuronidase and glucuronide permease gene system
    Patent number: 5599670
    Abstract: The present invention relates to the .beta.-glucuronidase (GUS) gene fusion system, and to the cloning and characterization of the .beta.-glucuronidase and glucuronide permease genes of Escherichia coli. It is based on the surprising discovery that gene fusions comprising the .beta.-glucuronidase gene may be effectively expressed in a wide variety of organisms to produce active .beta.-glucuronidase enzyme. Because of the abundance and availability of useful substrates for .beta.-glucuronidase enzyme, GUS gene fusions may serve as a superior reporter gene system as well as an effective means of altering cellular phenotype. In conjunction with recombinant glucuronide permease, which may be used to render host cells permeable to .beta.-glucuronidase substrates, the GUS gene fusion system offers almost unlimited applications in the fields of plant and animal genetic engineering.
    Type: Grant
    Filed: February 21, 1995
    Date of Patent: February 4, 1997
    Assignee: Cambia Biosystems. L.L.C.
    Inventor: Richard A. Jefferson
  • Host cells transformed with the E. coli glucoronide permease gene
    Patent number: 5432081
    Abstract: The present invention relates to the .beta.-glucuronidase (GUS) gene fusion system, and to the cloning and characterization of the .beta.-glucuronidase and glucuronide permease genes of Escherichia coli. It is based on the surprising discovery that gene fusions comprising the .beta.-glucuronidase gene may be effectively expressed in a wide variety of organisms to produce active .beta.-glucuronidase enzyme. Because of the abundance and availability of useful substrates for .beta.-glucuronidase enzyme, GUS gene fusions may serve as a superior reporter gene system as well as an effective means of altering cellular phenotype. In conjunction with recombinant glucuronide permease, which may be used to render host cells permeable to .beta.-glucuronidase substrates, the GUS gene fusion system offers almost unlimited applications in the fields of plant and animal genetic engineering.
    Type: Grant
    Filed: October 15, 1993
    Date of Patent: July 11, 1995
    Assignee: Cambia Biosystems, L.L.C.
    Inventor: Richard A. Jefferson