Abstract: Fluorophore-labeled collagen covalently bound to a cell culture vessel can be used to assay collagen degradation by a variety of cell types, including osteoclasts and tumor cells. Such assays provide a high throughput platform for rapid screening of large numbers of potential modulators of, for example, tumor metastasis or osteoclast differentiation and/or function.

Abstract: An apparatus and method for on-line testing for the presence of an endotoxin within a fluid sample from a fluid line. The apparatus is positioned in fluid communication with a fluid line to perform the on-line fluid testing for the presence of at least one endotoxin. The apparatus can include a housing and a fluid sampling system positioned in fluid communication with the fluid line. The fluid sampling system can comprise a valve for controlling the fluid flow from the fluid line into the fluid sampling system. A fluid flow well is positioned within the housing and in fluid communication with the fluid sampling system. A removable assembly can also be secured within the housing. The removable assembly comprises a plurality of wells for receiving used and unused fluid carrying members that can receive samples from the fluid flow well, a plurality of fluid sample receiving wells, and a plurality of vessel retention positions comprising recesses for securely receiving portions of respective fluid vessels.

Abstract: A reagent containing a purified horseshoe crab Factor C, particularly a recombinantly produced Factor C, and a surfactant can be used in a sensitive, rapid, and reproducible assay to detect endotoxin.