Abstract: The present disclosure provides systems and methods for sequencing nucleic acid molecules using tunneling labels. A sequence of a nucleic acid molecule may be identified with high accuracy using a chip comprising sensors, wherein each individual sensor may comprise at least two electrodes separated by a gap. The electrodes may be configured to generate at least one electrical signal upon binding of a tunneling label associated with a nucleotide. Epigenetic information can also be determined at the same time as a nucleic acid sequence.

Abstract: The disclosed embodiments concern methods, apparatus, systems and computer program products for determining the presence or absence of repeat expansions of interest, including repeat expansions of repeat sequences that are medically significant. Some embodiments provide methods for identifying and calling medically relevant repeat expansions using anchored reads. An anchored read is a paired end read that is unaligned to a repeat sequence under consideration, but it is paired with an anchor read that is aligned to or near the repeat sequence. Some embodiments use both anchor and anchored reads to determine the presence or absence of the repeat expansions. System, apparatus, and computer program products are also provided for determining repeat expansion implementing the methods disclosed.

Abstract: This invention relates to phototherapy lights, in particular for a combination of Bright Light Therapy and Dawn Simulation. A phototherapy light source having two operational modes, a first dawn simulator mode; and a second, bright light therapy mode; the light comprising a controller coupled to a light source; and wherein, in said first mode said light source is controlled to provide variable illumination with a minimum light intensity of less than 1 lux at 50 centimeters, and in said second mode said light source is controlled to provide illumination with a light intensity of at least 1000 lux at 50 centimeters.

Abstract: The invention relates to methods for indexing samples during the sequencing of polynucleotide templates, resulting in the attachment of tags specific to the source of each nucleic acid sample such that after a sequencing run, both the source and sequence of each polynucleotide can be determined. Thus, the present invention pertains to analysis of complex genomes (e.g., human genomes), as well as multiplexing less complex genomes, such as those of bacteria, viruses, mitochondria, and the like.

Abstract: The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a ligation reaction in which the first end of one or more target polynucleotide molecules are ligated to surface-bound adaptor polynucleotide molecules, and (b) a ligation reaction in which solution-phase adaptor polynucleotide molecules are ligated to the second end of said target polynucleotide molecules, in order to produce one or more template constructs attached to a solid support.

Abstract: The invention relates to methods for indexing samples during the sequencing of polynucleotide templates, resulting in the attachment of tags specific to the source of each nucleic acid sample such that after a sequencing run, both the source and sequence of each polynucleotide can be determined. Thus, the present invention pertains to analysis of complex genomes (e.g., human genomes), as well as multiplexing less complex genomes, such as those of bacteria, viruses, mitochondria, and the like.

Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.

Abstract: A technique is provided for classifying images in a library (1) for subsequent selective retrieval. Each library image is segmented (2) into a plurality of coherent regions. Each of the regions is analyzed in respect of a plurality of visual properties, each of which is ascribed a number according to the value of the visual property. The property numbers are then compared (4) with bands of the available property range and a computer-readable character, such as a letter of the alphabet is ascribed to each of the property values. The characters are then arranged (5) in a predetermined order to form a region character string. The strings for each region are then indexed (7) to form an index (8) of the images by region character string. For image retrieval, a target image (10) is analyzed in the same way to form region character strings which are then supplied to a text retrieval system (12) for detecting matches with entries in the index (8).

Abstract: Methods of reducing the power consumption of a microprocessor system, comprising a microprocessor and memory connected by a bus, are provided which involve optimising a program stored in the memory by rescheduling after linking, swapping equivalent instructions, or swapping the operands of commutative operations.

Abstract: A method for correcting distortions in multi-focus image stacks comprising the steps: acquiring a plurality of N colour images with an optical system; wherein each color image encompasses a plurality of pixels; converting each of two successive colour images of the N images into monochrome images; scaling each of the monochrome images to a conveniently M by M image; calculating a geometric transformation and a radiometric transformation of the two successive images, wherein the geometric transformation and the radiometric transformation is determined by a set of coefficients, which are determined by least square matching, and using the determined set of coefficients from each of two successive images, to correct each image sequentially and thereby constructing the multi-focus image by applying the determined parameter set to each of the successive images.

Abstract: A scraper for scraping live cells from a substrate. The scraper comprises a magnetic core and an elastomeric housing surrounding at least a portion of the magnetic core and defining at least one scraping blade. A method of scraping and a system for scraping are also provided.

Abstract: A cable 2 has an outer sheath on which a layer of selectively activatable material such as a layer of thick-film conductive polymer is sprayed to form a resistive element. A layer of thermochromic ink is printed on top of this. Electric current passed through the resistive element provides heating to the ink and causes it to warm and change color.

Abstract: A hydrophilic therapeutic agent is prepared in storage-stable form, suitable for administration to a patient. The agent is formulated with a hydrophobically-derivatized carbohydrate, making use of ion-pair formation to form a solution of the agent and carbohydrate.

Abstract: The invention encompasses compositions for increased bioavailability through mucosal delivery comprising, preferably in powder form, an intimate mixture of an effective amount of a bioactive agent and a hydrophobically derivatized carbohydrate. The compositions can also contain a surface active agent preferably for delivery to gastrointestinal mucosa and ocular delivery.