Abstract: Apparatus and methods are provided for the imaging of structures in deep tissue within biological specimens, using spectral imaging to provide highly sensitive detection. By acquiring data that provides a plurality of images of the sample with different spectral weightings, and subsequent spectral analysis, light emission from a target compound is separated from autofluorescence in the sample. With the autofluorescence reduced or eliminated, an improved measurement of the target compound is obtained.
Type:
Grant
Filed:
February 6, 2013
Date of Patent:
March 7, 2017
Assignee:
Cambridge Research & Intrumentation, Inc.