Abstract: Disclosed are compositions and methods for template-free nucleic acid synthesis using N-mers and/or anchor primers that comprise at least one XNA or a combination of RNA and DNA.
Type:
Grant
Filed:
January 13, 2020
Date of Patent:
March 31, 2026
Assignee:
CAMENA BIOSCIENCE LIMITED
Inventors:
Derek L. Stemple, Andrew G. Fraser, Sylwia Mankowska, Neil Bell
Abstract: Described herein are compositions of matter and methods to synthesize any nucleic acid (NA) sequence using completely natural nucleic acid sources without the need for large-scale phosphoramidite-mediated chemical synthesis.
Type:
Application
Filed:
April 26, 2022
Publication date:
June 27, 2024
Applicant:
CAMENA BIOSCIENCE LIMITED
Inventors:
Derek STEMPLE, Sylwia MANKOWSKA, Neil BELL
Abstract: The present disclosure provides compositions, kits and methods for sequencing double-stranded nucleic acids. The compositions, kits and methods comprise partially double-stranded identifier molecules and partially double-stranded adapter molecules. The compositions, kits and methods can be used to determine the abundance and/or identity of specific transcripts in a plurality of double-stranded nucleic acids, as well as identifying the frequency of mutations within certain transcripts in the plurality of double-stranded nucleic acids.